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TRIM72 抗体 (AA 28-477)

This anti-TRIM72 antibody is a 兔 多克隆 antibody detecting TRIM72 in WB, IHC, ELISA, FACS 和 IF. Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN7601079
发货至: 中国

Quick Overview for TRIM72 抗体 (AA 28-477) (ABIN7601079)

抗原

See all TRIM72 抗体
TRIM72 (Tripartite Motif Containing 72 (TRIM72))

适用

  • 26
  • 8
  • 7
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
人, 大鼠, 小鼠

宿主

  • 20
  • 5
  • 2

克隆类型

  • 24
  • 3
多克隆

标记

  • 20
  • 2
  • 2
  • 1
  • 1
  • 1
This TRIM72 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
  • 抗原表位

    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 28-477

    原理

    Anti-MG53/TRIM72 Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-MG53/TRIM72 Antibody Picoband® (ABIN7601079). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human MG53/TRIM72 recombinant protein (Position: E28-A477).

    亚型

    IgG
  • 应用备注

    Western blot, 0.25-0.5 μg/mL, Mouse, Rat
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
    Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, M., Hwang, M., Ko, J.-K., Lin, P., Thornton, A., Zhao, X., Pan, Z., Komazaki, S., Brotto, M., Takeshima, H., Ma, J. MG53 nucleates assembly of cell membrane repair machinery. Nature Cell Biol. 11: 56-64, 2009. 2. Song, R., Peng, W., Zhang, Y., Lv, F., Wu, H.-K., Guo, J., Cao, Y., Pi, Y., Zhang, X., Jin, L., Zhang, M., Jiang, P., Liu, F., Meng, S., Zhang, X., Jiang, P., Cao, C.-M., Xiao, R.-P. Central role of E3 ubiquitin ligase MG53 in insulin resistance and metabolic disorders. Nature 494: 375-379, 2013. 3. Zhu, H., Lin, P., De, G., Choi, K., Takeshima, H., Weisleder, N., Ma, J. Polymerase transcriptase release factor (PTRF) anchors MG53 protein to cell injury site for initiation of membrane repair. J. Biol. Chem. 286: 12820-12824, 2011.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    TRIM72 (Tripartite Motif Containing 72 (TRIM72))

    别名

    TRIM72

    背景

    Synonyms: Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, AFB1-AR 1, Aldoketoreductase 7, Succinic semialdehyde reductase, SSA reductase, AKR7A2, AFAR, AFAR1, AKR7

    Tissue Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.

    Background: Enables identical protein binding activity. Predicted to be involved in several processes, including cellular protein metabolic process, plasma membrane repair, and protein homooligomerization. Predicted to act upstream of or within negative regulation of insulin receptor signaling pathway, negative regulation of insulin-like growth factor receptor signaling pathway, and negative regulation of myotube differentiation. Predicted to be located in cytoplasmic vesicle membrane. Predicted to be active in cytoplasm and sarcolemma.

    分子量

    55 kDa

    基因ID

    493829
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