NAD-ME 抗体 (AA 26-584)
Quick Overview for NAD-ME 抗体 (AA 26-584) (ABIN7600967)
抗原
See all NAD-ME 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 26-584
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原理
- Anti-ME2 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-ME2 Antibody Picoband® (ABIN7600967). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human ME2 recombinant protein (Position: K26-E584).
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Burchell, A., Crosby, A., Cohen, P. T. W. Human mitochondrial malic enzyme variants: properties of the different polymorphic forms. Ann. Hum. Genet. 41: 1-7, 1977. 2. Champion, M. J., Brown, J. A., Shows, T. B. Assignment of cytoplasmic alpha-mannosidase (MAN-A) and confirmation of mitochondrial isocitrate dehydrogenase (IDH-M) to the q11-qter region of chromosome 15 in man. Cytogenet. Cell Genet. 22: 498-502, 1978. 3. Cohen, P. T. W., Omenn, G. S. Genetic variation of the cytoplasmic and mitochondrial malic enzymes in the monkey: Macaca nemestrina. Biochem. Genet. 7: 289-301, 1972. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))
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别名
- ME2
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背景
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Synonyms: NAD-dependent malic enzyme, mitochondrial, NAD-ME, Malic enzyme 2, ME2
Tissue Specificity: Ubiquitous. Highly expressed in testis.
Background: NAD-dependent malic enzyme, mitochondrial is a protein that in humans is encoded by the ME2 gene. It is mapped to 18q21.2. This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.
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分子量
- 65 kDa
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基因ID
- 4200
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UniProt
- P23368
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途径
- Production of Molecular Mediator of Immune Response
抗原
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