SH3KBP1 抗体 (AA 256-629)
Quick Overview for SH3KBP1 抗体 (AA 256-629) (ABIN7600938)
抗原
See all SH3KBP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 256-629
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原理
- Anti-SH3KBP1 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-SH3KBP1 Antibody Picoband® (ABIN7600938). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SH3KBP1 recombinant protein (Position: D256-Q629).
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Feng, L., Wang, J.-T., Jin, H., Qian, K., Geng, J.-G. SH3KBP1-binding protein 1 prevents epidermal growth factor receptor degradation by the interruption of c-Cbl-CIN85 complex. Cell Biochem. Funct. 29: 589-596, 2011. 2. Keller, B., Shoukier, M., Schulz, K., Bhatt, A., Heine, I., Strohmeier, V., Speckmann, C., Engels, N., Warnatz, K., Wienands, J. Germline deletion of CIN85 in humans with X chromosome-linked antibody deficiency. J. Exp. Med. 215: 1327-1336, 2018. 3. Kometani, K., Yamada, T., Sasaki, Y., Yokosuka, T., Saito, T., Rajewsky, K., Ishiai, M., Hikida, M., Kurosaki, T. CIN85 drives B cell responses by linking BCR signals to the canonical NF-kappaB pathway. J. Exp. Med. 208: 1447-1457, 2011. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SH3KBP1 (SH3-Domain Kinase Binding Protein 1 (SH3KBP1))
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别名
- SH3KBP1
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背景
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Synonyms: Elongator complex protein 3, Help3, ELP3
Tissue Specificity: Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level). Also expressed in jejunum and duodenum.
Background: SH3 domain-containing kinase-binding protein 1 is an adaptor protein that in humans is encoded by the SH3KBP1 gene. This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms.
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分子量
- 85 kDa
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基因ID
- 30011
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UniProt
- Q96B97
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途径
- EGFR Signaling Pathway, EGFR Downregulation
抗原
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