ZFP42 抗体 (AA 25-310)
Quick Overview for ZFP42 抗体 (AA 25-310) (ABIN7600901)
抗原
See all ZFP42 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 25-310
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原理
- Anti-Rex1/ZFP42 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Rex1/ZFP42 Antibody Picoband® (ABIN7600901). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Rex1/ZFP42 recombinant protein (Position: A25-K310).
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亚型
- IgG
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anti-Zinc Finger Protein 42 (ZFP42) (AA 249-310) antibody
ZFP42 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5E11A6 unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (AA 249-310) antibodyZFP42 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (AA 6-68) antibodyZFP42 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (AA 1-310) antibodyZFP42 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (N-Term) antibodyZFP42 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (AA 50-256) antibodyZFP42 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (C-Term) antibodyZFP42 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (AA 108-157) antibodyZFP42 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (N-Term) antibodyZFP42 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 42 (ZFP42) (AA 1-140) antibodyZFP42 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gontan, C., Achame, E. M., Demmers, J., Barakat, T. S., Rentmeester, E., van IJcken, W., Grootegoed, J. A., Gribnau, J. RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation. Nature 485: 386-390, 2012. 2. Henderson, J. K., Draper, J. S., Baillie, H. S., Fishel, S., Thomson, J. A., Moore, H., Andrews, P. W. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337, 2002. 3. Hosler, B. A., LaRosa, G. J., Grippo, J. F., Gudas, L. J. Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells. Molec. Cell. Biol. 9: 5623-5629, 1989. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ZFP42 (Zinc Finger Protein 42 (ZFP42))
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别名
- ZFP42
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背景
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Synonyms: Zinc finger protein 42 homolog, Zfp-42, Reduced expression protein 1, REX-1, hREX-1, Zinc finger protein 754, ZFP42, REX1, ZNF754
Tissue Specificity: Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.
Background: Rex1 is a protein that in humans is encoded by the ZFP42 gene. It is mapped to 4q35.2. Rex1 (Zfp-42) is a known marker of pluripotency, and is usually found in undifferentiated embryonic stem cells. In addition to being a marker for pluripotency, its regulation is also critical in maintaining a pluripotent state. As the cells begin to differentiate, Rex1 is severely and abruptly downregulated.
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分子量
- 45 kDa
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基因ID
- 132625
抗原
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