PDE6B 抗体 (AA 25-237)
Quick Overview for PDE6B 抗体 (AA 25-237) (ABIN7600894)
抗原
See all PDE6B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 25-237
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原理
- Anti-PDE6 beta/PDE6B Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PDE6 beta/PDE6B Antibody Picoband® (ABIN7600894). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PDE6 beta/PDE6B recombinant protein (Position: K25-Q237).
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亚型
- IgG
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anti-phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B) (AA 1-190) antibody
PDE6B 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B) antibodyPDE6B 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B) (AA 1-853) antibodyPDE6B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B) (AA 105-154) antibodyPDE6B 适用: 人, Cow, 犬, 马, 豚鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B) (AA 372-421) antibodyPDE6B 适用: 人, Cow, 犬, 马 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hmani-Aifa, M., Benzina, Z., Zulfiqar, F., Dhouib, H., Shahzadi, A., Ghorbel, A., Rebai, A., Soderkvist, P., Riazuddin, S., Kimberling, W. J., Ayadi, H. Identification of two new mutations in the GPR98 and the PDE6B genes segregating in a Tunisian family. Europ. J. Hum. Genet. 17: 474-482, 2009. 2. Tsang, S. H., Woodruff, M. L., Jun, L., Mahajan, V., Yamashita, C. K., Pedersen, R., Lin, C.-S., Goff, S. P., Rosenberg, T., Larsen, M., Farber, D. B., Nusinowitz, S. Transgenic mice carrying the H258N mutation in the gene encoding the beta-subunit of phosphodiesterase-6 (PDE6B) provide a model for human congenital stationary night blindness. Hum. Mutat. 28: 243-254, 2007. 3. Zhao, L., Zabel, M. K., Wang, X., Ma, W., Shah, P., Fariss, R. N., Qian, H., Parkhurst, C. N., Gan, W.-B., Wong, W. T. Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Molec. Med. 7: 1179-1197, 2015. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))
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别名
- PDE6B
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背景
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Synonyms: Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT
Tissue Specificity: Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.
Background: Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
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分子量
- 98 kDa
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基因ID
- 5158
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UniProt
- P35913
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途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
抗原
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