U2AF2 抗体 (AA 238-470)
Quick Overview for U2AF2 抗体 (AA 238-470) (ABIN7600794)
抗原
See all U2AF2 (U2AF59) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 238-470
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原理
- Anti-U2AF65/U2AF2 Picoband® Antibody (monoclonal, 10F4)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-U2AF65/U2AF2 Picoband® Antibody (monoclonal, 10F4) (ABIN7600794). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human U2AF65/U2AF2 recombinant protein (Position: M238-H470).
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亚型
- IgG2b
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anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) (AA 192-471) antibody
U2AF59 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) (AA 141-240) antibodyU2AF59 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5G8 unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) antibodyU2AF59 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) (N-Term) antibodyU2AF59 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) antibodyU2AF59 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) (AA 124-246) antibodyU2AF59 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) (N-Term) antibodyU2AF59 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 豚鼠, 斑马鱼, 非洲爪蟾, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) (AA 371-420) antibodyU2AF59 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 豚鼠, 斑马鱼, 猴, Pig, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) (N-Term) antibodyU2AF59 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59) (C-Term) antibodyU2AF59 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Kielkopf, C. L., Rodionova, N. A., Green, M. R., Burley, S. K. A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer. Cell 106: 595-605, 2001. 2. Mackereth, C. D., Madl, T., Bonnal, S., Simon, B., Zanier, K., Gasch, A., Rybin, V., Valcarcel, J., Sattler, M. Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF. Nature 475: 408-411, 2011. 3. Sickmier, E. A., Frato, K. E., Shen, H., Paranawithana, S. R., Green, M. R., Kielkopf, C. L. Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65. Molec. Cell 23: 49-59, 2006. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))
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别名
- U2AF2
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背景
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Synonyms: Eukaryotic translation initiation factor 6, eIF-6, B (2)GCN homolog, B4 integrin interactor, CAB, p27 (BBP), EIF6, EIF3A, ITGB4BP, OK/SW-cl.27
Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.
Background: Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
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分子量
- 65 kDa
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基因ID
- 11338
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UniProt
- P26368
抗原
-
