MFGE8 抗体 (AA 23-463)
Quick Overview for MFGE8 抗体 (AA 23-463) (ABIN7600756)
抗原
See all MFGE8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 23-463
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原理
- Anti-Milk Fat Globule 1/Mfge8 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Milk Fat Globule 1/Mfge8 Antibody Picoband® (ABIN7600756). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived mouse Milk Fat Globule 1/Mfge8 recombinant protein (Position: A23-C463). Mouse?Mfge8 shares?86.2%?amino?acid?(aa)?sequence?identity?with rat Mfge8.
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亚型
- IgG
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anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) (AA 1-200) antibody
MFGE8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibodyMFGE8 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal MFG-06 unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) (AA 159-417) antibodyMFGE8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) (AA 73-421) antibodyMFGE8 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibodyMFGE8 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibodyMFGE8 适用: 人 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal EDM45 unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibodyMFGE8 适用: 人, 犬 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal MFG-06 unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibodyMFGE8 适用: 人 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM291 unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) (AA 25-334) antibodyMFGE8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibodyMFGE8 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2B2D10 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Bu, H.-F., Zuo, X.-L., Wang, X., Ensslin, M. A., Koti, V., Hsueh, W., Raymond, A. S., Shur, B. D., Tan, X.-D. Milk fat globule-EGF factor 8/lactadherin plays a crucial role in maintenance and repair of murine intestinal epithelium. J. Clin. Invest. 117: 3673-3683, 2007. 2. Collins, C., Nehlin, J. O., Stubbs, J. D., Kowbel, D., Kuo, W.-L., Parry, G. Mapping of a newly discovered human gene homologous to the apoptosis associated-murine mammary protein, MFG-E8, to chromosome 15q25. Genomics 39: 117-118, 1997. 3. Hanayama, R., Tanaka, M., Miwa, K., Shinohara, A., Iwamatsu, A., Nagata, S. Identification of a factor that links apoptotic cells to phagocytes. Nature 417: 182-187, 2002. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
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别名
- Mfge8
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背景
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Synonyms: Tumor necrosis factor receptor superfamily member 4, MRC OX40, OX40 antigen, OX40L receptor, CD134, Tnfrsf4, Ox40, Txgp1l
Background: MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al. (1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al. (2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al. (2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
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分子量
- 65-70 kDa
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基因ID
- 17304
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UniProt
- P21956
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途径
- SARS-CoV-2 Protein Interactome
抗原
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