NUP133 抗体 (AA 228-1156)
Quick Overview for NUP133 抗体 (AA 228-1156) (ABIN7600723)
抗原
See all NUP133 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 228-1156
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原理
- Anti-NUP133 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-N Antibody Picoband® (ABIN7600723). Tested in ELISA, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NUP133 recombinant protein (Position: Q228-I1156).
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亚型
- IgG
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anti-Nucleoporin 133kDa (NUP133) antibody
NUP133 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 133kDa (NUP133) (AA 1069-1155) antibodyNUP133 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3E8 unconjugated
anti-Nucleoporin 133kDa (NUP133) (AA 36-240) antibodyNUP133 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 133kDa (NUP133) (AA 1069-1156) antibodyNUP133 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3E8 unconjugated
anti-Nucleoporin 133kDa (NUP133) (AA 1069-1155) antibodyNUP133 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F6 unconjugated
anti-Nucleoporin 133kDa (NUP133) (AA 899-1091) antibodyNUP133 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 133kDa (NUP133) (AA 730-930) antibodyNUP133 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human, Rat
Immunoprecipitation, 0.5-2 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Belgareh, N., Rabut, G., Bai, S. W., van Overbeek, M., Beaudouin, J., Daigle, N., Zatsepina, O. V., Pasteau, F., Labas, V., Fromont-Racine, M., Ellenberg, J., Doye, V. An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. J. Cell. Biol. 154: 1147-1160, 2001. 2. Braun, D. A., Lovric, S., Schapiro, D., Schneider, R., Marquez, J., Asif, M., Hussain, M. S., Daga, A., Widneier, E., Rao, J., Ashraf, S., Tan, W., and 46 others. Mutations in multiple components of the nuclear pore complex cause nephrotic syndrome. J. Clin. Invest. 128: 4313-4328, 2018. 3. Fujita, A., Tsukaguchi, H., Koshimizu, E., Nakazato, H., Itoh, K., Kuraoka, S., Komohara, Y., Shiina, M., Nakamura, S., Kitajima, M., Tsurusaki, Y., Miyatake, S., Ogata, K., Iijima, K., Matsumoto, N., Miyake, N. Homozygous splicing mutation in NUP133 causes Galloway-Mowat syndrome. Ann. Neurol. 84: 814-828, 2018. Note: Erratum: Ann. Neurol. 85: 462-463, 2019. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NUP133 (Nucleoporin 133kDa (NUP133))
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别名
- NUP133
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背景
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Synonyms: Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG, CKAP5, KIAA0097
Tissue Specificity: Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. Expression is elevated in the brain, highly expressed in the Purkinje cell bodies of the cerebellum.
Background: Nuclear pore complex protein Nup133, or Nucleoporin Nup133, is a protein that in humans is encoded by the NUP133 gene. The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
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分子量
- 129 kDa
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基因ID
- 55746
抗原
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