PPP1R15B 抗体 (AA 211-657)
Quick Overview for PPP1R15B 抗体 (AA 211-657) (ABIN7600639)
抗原
See all PPP1R15B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 211-657
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原理
- Anti-PPP1R15B Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-PPP1R15B Antibody Picoband® (ABIN7600639). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PPP1R15B recombinant protein (Position: Q211-D657).
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亚型
- IgG
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anti-Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B) (Internal Region) antibody
PPP1R15B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B) (AA 480-529) antibodyPPP1R15B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B) (AA 494-522), (C-Term) antibodyPPP1R15B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32305 unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B) (Internal Region) antibodyPPP1R15B 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Harding, H. P., Zhang, Y., Scheuner, D., Chen, J.-J., Kaufman, R. J., Ron, D. Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2-alpha) dephosphorylation in mammalian development. Proc. Nat. Acad. Sci. 106: 1832-1837, 2009. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))
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别名
- PPP1R15B
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背景
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Synonyms: Protein NDRG3,N-myc downstream-regulated gene 3 protein,NDRG3,
Tissue Specificity: Ubiquitous. Highly expressed in brain. .
Background: PPP1R15B(Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1(PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of thePPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -/- mice at a mendelian ratio. However, Ppp1r15b -/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -/- embryos with wildtype Eif2-alpha.
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分子量
- 110 kDa
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基因ID
- 84919
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途径
- ER-Nucleus Signaling
抗原
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