MLKL 抗体 (AA 210-464)
Quick Overview for MLKL 抗体 (AA 210-464) (ABIN7600633)
抗原
See all MLKL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 210-464
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原理
- Anti-MLKL Antibody Picoband®
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交叉反应 （详细）
- No cross reactivity with other proteins.
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产品特性
- Anti-MLKL Antibody Picoband® (ABIN7600633). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human MLKL recombinant protein (Position: R210-K464). Human MLKL shares 69.7% amino acid (aa) sequence identity with mouse MLKL.
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亚型
- IgG
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anti-Mixed Lineage Kinase Domain-Like (MLKL) (AA 371-471) antibody
MLKL 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B2 unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) (Internal Region) antibodyMLKL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) (AA 444-472) antibodyMLKL 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) (AA 1-471) antibodyMLKL 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) (pSer358) antibodyMLKL 适用: 人 IHC (p) 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) antibodyMLKL 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) antibodyMLKL 适用: 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) antibodyMLKL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) (AA 444-472), (C-Term) antibodyMLKL 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mixed Lineage Kinase Domain-Like (MLKL) (N-Term) antibodyMLKL 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dastani, Z., Pajukanta, P., Marcil, M., Rudzicz, N., Ruel, I., Bailey, S. D., Lee, J. C., Lemire, M., Faith, J., Platko, J., Rioux, J., Hudson, T. J., Gaudet, D., Engert, J. C., Genest, J. Fine mapping and association studies of a high-density lipoprotein cholesterol linkage region on chromosome 16 in French-Canadian subjects. Europ. J. Hum. Genet. 18: 342-347, 2010. 2. Sun, L., Wang, H., Wang, Z., He, S., Chen, S., Liao, D., Wang, L., Yan, J., Liu, W., Lei, X., Wang, X. Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148: 213-227, 2012. 3. Wang, Z., Jiang, H., Chen, S., Du, F., Wang, X. The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways. Cell 148: 228-243, 2012. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- MLKL (Mixed Lineage Kinase Domain-Like (MLKL))
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别名
- MLKL
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背景
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Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Tissue Specificity: Expressed in all tissues.
Background: Mixed lineage kinase domain like pseudokinase (MLKL) is a protein that in humans is encoded by the MLKL gene. This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain, however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
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分子量
- 50 kDa
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基因ID
- 197259
抗原
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