ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
原理
Anti-MEST Antibody Picoband®
产品特性
Anti-MEST Antibody Picoband® (ABIN7600611). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
纯化方法
Immunogen affinity purified.
免疫原
E.coli-derived human MEST recombinant protein (Position: A21-D320). Human MEST shares 97.7% and 98.3% amino acid (aa) sequence identity with mouse and rat MEST, respectively.
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, - 1. Decker, M., Adamska, M., Cronin, A., Di Giallonardo, F., Burgener, J., Marowsky, A., Falck, J. R., Morisseau, C., Hammock, B. D., Gruzdev, A., Zeldin, D. C., Arand, M. EH3 (ABHD9): the first member of a new epoxide hydrolase family with high activity for fatty acid epoxides. J. Lipid Res. 53: 2038-2045, 2012. 2. Ferguson-Smith, A. C., Cattanach, B. M., Barton, S. C., Beechey, C. V., Surani, M. A. Embryological and molecular investigations of parental imprinting on mouse chromosome 7. Nature 351: 667-670, 1991. 3. Kaneko-Ishino, T., Kuroiwa, Y., Miyoshi, N., Kohda, T., Suzuki, R., Yokoyama, M., Viville, S., Barton, S. C., Ishino, F., Surani, M. A. Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization. Nature Genet. 11: 52-59, 1995.
限制
仅限研究用
状态
Lyophilized
溶解方式
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Mesoderm-specific transcript homolog protein is a protein that in humans is encoded by the MEST gene. This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.