MPI 抗体 (AA 2-99)
Quick Overview for MPI 抗体 (AA 2-99) (ABIN7600514)
抗原
See all MPI 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 2-99
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原理
- Anti-MPI Picoband® Antibody (monoclonal, 5G5)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MPI Picoband® Antibody (monoclonal, 5G5) (ABIN7600514). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.
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亚型
- IgG2a
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anti-Mannose Phosphate Isomerase (MPI) (AA 2-423) antibody
MPI 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mannose Phosphate Isomerase (MPI) antibodyMPI 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1C7 unconjugated
anti-Mannose Phosphate Isomerase (MPI) (AA 1-423) antibodyMPI 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mannose Phosphate Isomerase (MPI) antibodyKO Validated MPI 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mannose Phosphate Isomerase (MPI) (AA 213-350) antibodyMPI 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mannose Phosphate Isomerase (MPI) (AA 1-423) antibodyMPI 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Jaeken, J., Matthijs, G., Saudubray, J.-M., Dionisi-Vici, C., Bertini, E., de Lonlay, P., Henri, H., Carchon, H., Schollen, E., Van Schaftingen, E. Phosphomannose isomerase deficiency: a carbohydrate-deficient glycoprotein syndrome with hepatic-intestinal presentation. (Letter) Am. J. Hum. Genet. 62: 1535-1539, 1998. 2. Schollen, E., Dorland, L., de Koning, T. J., Van Diggelen, O. P., Huijmans, J. G. M., Marquardt, T., Babovic-Vuksanovic, D., Patterson, M., Imtiaz, F., Winchester, B., Adamowicz, M., Pronicka, E., Freeze, H., Matthijs, G. Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib). Hum. Mutat. 16: 247-252, 2000. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MPI (Mannose Phosphate Isomerase (MPI))
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别名
- MPI
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背景
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Synonyms: Mannose-6-phosphate isomerase, Phosphohexomutase, Phosphomannose isomerase, PMI, MPI, PMI1
Tissue Specificity: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
Background: Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.
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分子量
- 45 kDa
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基因ID
- 4351
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UniProt
- P34949
抗原
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