ALT 抗体 (AA 2-496)
Quick Overview for ALT 抗体 (AA 2-496) (ABIN7600506)
抗原
See all ALT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-496
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原理
- Anti-Alanine Transaminase/Gpt Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-Alanine Transaminase/Gpt Antibody Picoband® (ABIN7600506). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived mouse Alanine Transaminase/Gpt recombinant protein (Position: A2-S496).
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Astrin, K. H., Arredondo-Vega, F. X., Desnick, R. J., Smith, M. Assignment of the gene for cytosolic alanine aminotransferase (AAT1) to human chromosome 8. Ann. Hum. Genet. 46: 125-133, 1982. 2. Chen, S.-H., Giblett, E. R. Polymorphism of soluble glutamic-pyruvic transaminase: a new genetic marker in man. Science 173: 148-149, 1971. 3. Cook, P. J. L., Jeremiah, S. J., Buckton, K. E. Exclusion mapping of GPT. (Abstract) Cytogenet. Cell Genet. 32: 258 only, 1982. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ALT (Alanine Aminotransferase (ALT))
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别名
- Gpt
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背景
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Synonyms: Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin, PITX2, ARP1, RGS, RIEG, RIEG1
Tissue Specificity: Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.
Background: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1), also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase
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分子量
- 55 kDa
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基因ID
- 76282
抗原
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