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ALT 抗体 (AA 2-496)

This anti-ALT antibody is a 兔 多克隆 antibody detecting ALT in WB, IHC, ELISA, ICC, IF 和 FACS. Suitable for 大鼠 和 小鼠.
产品编号 ABIN7600506
发货至: 中国

Quick Overview for ALT 抗体 (AA 2-496) (ABIN7600506)

抗原

See all ALT 抗体
ALT (Alanine Aminotransferase (ALT))

适用

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大鼠, 小鼠

宿主

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克隆类型

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多克隆

标记

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This ALT antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 抗原表位

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    AA 2-496

    原理

    Anti-Alanine Transaminase/Gpt Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-Alanine Transaminase/Gpt Antibody Picoband® (ABIN7600506). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived mouse Alanine Transaminase/Gpt recombinant protein (Position: A2-S496).

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.25 μg/mL, Mouse, Rat
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
    ELISA, 0.1-0.5 μg/mL, -
    1. Astrin, K. H., Arredondo-Vega, F. X., Desnick, R. J., Smith, M. Assignment of the gene for cytosolic alanine aminotransferase (AAT1) to human chromosome 8. Ann. Hum. Genet. 46: 125-133, 1982. 2. Chen, S.-H., Giblett, E. R. Polymorphism of soluble glutamic-pyruvic transaminase: a new genetic marker in man. Science 173: 148-149, 1971. 3. Cook, P. J. L., Jeremiah, S. J., Buckton, K. E. Exclusion mapping of GPT. (Abstract) Cytogenet. Cell Genet. 32: 258 only, 1982.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    ALT (Alanine Aminotransferase (ALT))

    别名

    Gpt

    背景

    Synonyms: Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin, PITX2, ARP1, RGS, RIEG, RIEG1

    Tissue Specificity: Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.

    Background: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1), also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase

    分子量

    55 kDa

    基因ID

    76282
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