SRP54 抗体 (AA 2-433)
Quick Overview for SRP54 抗体 (AA 2-433) (ABIN7600501)
抗原
See all SRP54 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-433
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原理
- Anti-SRP54 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SRP54 Antibody Picoband® (ABIN7600501). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SRP54 recombinant protein (Position: V2-K433).
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亚型
- IgG
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anti-Signal Recognition Particle 54kDa (SRP54) antibody
SRP54 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) (AA 1-504) antibodySRP54 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) (AA 346-374) antibodySRP54 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) (AA 1-100) antibodySRP54 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 2G7 unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) (AA 196-455) antibodySRP54 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) antibodySRP54 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) (AA 391-440) antibodySRP54 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) (AA 182-231) antibodySRP54 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼, Bat, 小鸡, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) (Middle Region) antibodySRP54 适用: 人, 小鼠, 大鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle 54kDa (SRP54) (Middle Region) antibodySRP54 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼, 山羊 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bellanne-Chantelot, C., Schmaltz-Panneau, B., Marty, C., Fenneteau, O., Callebaut, I., Clauin, S., Docet, A., Damaj, G.-L., Leblanc, T., Pellier, I., Stoven, C., Souquere, S., and 26 others. Mutations in the SRP54 gene cause severe congenital neutropenia as well as Shwachman-Diamond-like syndrome. Blood 132: 1318-1331, 2018. 2. Carapito, R., Konantz, M., Paillard, C., Miao, Z., Pichot, A., Leduc, M. S., Yang, Y., Bergstrom, K. L., Mahoney, D. H., Shardy, D. L., Alsaleh, G., Naegely, L., and 38 others. Mutations in signal recognition particle SRP54 cause syndromic neutropenia with Shwachman-Diamond-like features. J. Clin. Invest. 127: 4090-4103, 2017. 3. Gowda, K., Chittenden, K., Zwieb, C. Binding site of the M-domain of human protein SRP54 determined by systematic site-ed mutagenesis of signal recognition particle RNA. Nucleic Acids Res. 25: 388-394, 1997. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SRP54 (Signal Recognition Particle 54kDa (SRP54))
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别名
- SRP54
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背景
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Synonyms: Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP25, USP21
Tissue Specificity: Isoform USP25a is found in most adult and fetal tissues, expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.
Background: The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).
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分子量
- 256 kDa
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基因ID
- 6729
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UniProt
- P61011
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途径
- SARS-CoV-2 Protein Interactome
抗原
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