EIF1 抗体 (AA 2-10)
Quick Overview for EIF1 抗体 (AA 2-10) (ABIN7600461)
抗原
See all EIF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-10
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原理
- Anti-EIF1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EIF1 Antibody Picoband® (ABIN7600461). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EIF1 recombinant protein (Position: S2-D106).
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 1 (EIF1) (AA 100-133), (C-Term) antibody
EIF1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB43570 unconjugated
anti-Eukaryotic Translation Initiation Factor 1 (EIF1) (AA 2-113) antibodyEIF1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 1 (EIF1) (AA 31-61) antibodyEIF1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB54642 unconjugated
anti-Eukaryotic Translation Initiation Factor 1 (EIF1) (AA 1-113) antibodyEIF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E1 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Sheikh M.S., Fernandez-Salas E., Yu M., Hussain A., Fornace A.J. Jr. J. Biol. Chem. 274:16487-16493(1999) 2. Mendell J.T., Medghalchi S.M., Lake R.G., Noensie E.N., Dietz H.C. Mol. Cell. Biol. 20:8944-8957(2000) -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EIF1 (Eukaryotic Translation Initiation Factor 1 (EIF1))
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别名
- EIF1
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背景
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Synonyms: Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hGC-1, hOLfD, OLFM4, GW112, UNQ362, PRO698
Tissue Specificity: Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues.
Background: Eukaryotic translation initiation factor 1 (eIF1) is a protein that in humans is encoded by the EIF1 gene. It is related to yeast SUI1. In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro. During the scanning process, eIF1 is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, 48S and 40S. eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. It is also a promotor, along with eukar-yotic translation initiation factor 1A (eIF1A), for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA. In addition, eIF1 and eIF1A, together with eukaryotic translation initiation factor 5 (eIF5), function in the formation of stable 40S ribosomal preinitiation complexes.
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分子量
- 13 kDa
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基因ID
- 10209
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UniProt
- P41567
抗原
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