CIAO3 (AA 196-476) 抗体
Quick Overview for CIAO3 (AA 196-476) 抗体 (ABIN7600446)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 196-476
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原理
- Anti-NARFL/CIAO3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-NARFL-CIAO3 Antibody Picoband® (ABIN7600446). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NARFL/CIAO3 recombinant protein (Position: Y196-W476). Human NARFL/CIAO3 shares 88.3% and 86.8% amino acid (aa) sequence identity with mouse and rat NARFL/CIAO3, respectively.
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亚型
- IgG
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anti-CIAO3 (AA 2-265) antibody
适用: 人 ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/19/2011. 2. Huang, J., Song, D., Flores, A., Zhao, Q., Mooney, S. M., Shaw, L. M., Lee, F. S. IOP1, a novel hydrogenase-like protein that modulates hypoxia-inducible factor-1-alpha activity. Biochem. J. 401: 341-352, 2007. 3. Song, D., Lee, F. S. Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. J. Biol. Chem. 286: 15797-15805, 2011. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CIAO3
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背景
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Synonyms: CIAO3, NARFL, PRN, Cytosolic iron-sulfur assembly component 3, Cytosolic Fe-S cluster assembly factor NARFL, Iron-only hydrogenase-like protein 1, IOP1, Nuclear prelamin A recognition factor-like protein, Protein related to Narf
Background: Predicted to enable 4 iron, 4 sulfur cluster binding activity and metal ion binding activity. Involved in several processes, including intracellular oxygen homeostasis, iron-sulfur cluster assembly, and response to hypoxia. Part of cytosolic [4Fe-4S] assembly targeting complex.
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分子量
- 53 kDa
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基因ID
- 64428
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UniProt
- Q9H6Q4
抗原
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