DOCK4 抗体 (AA 191-1837)
Quick Overview for DOCK4 抗体 (AA 191-1837) (ABIN7600431)
抗原
See all DOCK4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 191-1837
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原理
- Anti-DOCK4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DOCK4 Antibody Picoband® (ABIN7600431). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DOCK4 recombinant protein (Position: K191-I1837).
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亚型
- IgG
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anti-Dedicator of Cytokinesis 4 (DOCK4) (AA 1867-1966) antibody
DOCK4 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 3E7 unconjugated
anti-Dedicator of Cytokinesis 4 (DOCK4) (AA 1867-1966) antibodyDOCK4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B3 unconjugated
anti-Dedicator of Cytokinesis 4 (DOCK4) (AA 1-300) antibodyDOCK4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dedicator of Cytokinesis 4 (DOCK4) (AA 1867-1966) antibodyDOCK4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Dedicator of Cytokinesis 4 (DOCK4) (AA 150-450) antibodyDOCK4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hiramoto-Yamaki, N., Takeuchi, S., Ueda, S., Harada, K., Fujimoto, S., Negishi, M. Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism. J. Cell Biol. 190: 461-477, 2010. 2. Huang, L., Chambliss, K. L., Gao, X., Yuhanna, I. S., Behling-Kelly, E., Bergaya, S., Ahmed, M., Michaely, P., Luby-Phelps, K., Darehshouri, A., Xu, L., Fisher, E. A., Ge, W.-P., Mineo, C., Shaul, P. W. SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis. Nature 569: 565-569, 2019. 3. Kobayashi, M., Harada, K., Negishi, M., Katoh, H. Dock4 forms a complex with SH3YL1 and regulates cancer cell migration. Cell. Signal. 26: 1082-1088, 2014. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- DOCK4 (Dedicator of Cytokinesis 4 (DOCK4))
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别名
- DOCK4
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背景
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Synonyms: B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-like protein, Protein BCL9-2, BCL9L, DLNB11
Tissue Specificity: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.
Background: This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized.
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分子量
- 250 kDa
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基因ID
- 9732
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UniProt
- Q8N1I0
抗原
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