NUB1 抗体 (AA 190-543)
Quick Overview for NUB1 抗体 (AA 190-543) (ABIN7600427)
抗原
See all NUB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 190-543
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原理
- Anti-NUB1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NUB1 Antibody Picoband® (ABIN7600427). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NUB1 recombinant protein (Position: K190-D543).
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亚型
- IgG
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anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (AA 1-326) antibody
NUB1 适用: 人, 猴, 大鼠, 犬 WB, IHC, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 4H2 unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) antibodyNUB1 适用: 人, 小鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (AA 1-326) antibodyNUB1 适用: 人 WB, IHC, IHC (p), FACS 宿主: 小鼠 Monoclonal 2G1 unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (C-Term) antibodyNUB1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (AA 87-117), (N-Term) antibodyNUB1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2874 unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (AA 1-601) antibodyNUB1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (C-Term) antibodyNUB1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (AA 513-542), (C-Term) antibodyNUB1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2875 unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (C-Term) antibodyNUB1 适用: 人, 小鼠, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1) (AA 566-615) antibodyNUB1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Akey, D. T., Zhu, X., Dyer, M., Li, A., Sorensen, A., Blackshaw,S., Fukuda-Kamitani, T., Daiger, S. P., Craft, C. M., Kamitani, T., Sohocki, M. M. The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1. Hum. Molec. Genet. 11: 2723-2733, 2002. Note: Erratum: Hum. Molec. Genet. 12: 451 only, 2003. 2. Kito, K., Yeh, E. T. H., Kamitani, T. NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression. J. Biol. Chem. 276: 20603-20609, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))
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别名
- NUB1
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背景
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Synonyms: RNA-binding protein Nova-1, Neuro-oncological ventral antigen 1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1, NOVA1
Tissue Specificity: Expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney.
Background: NEDD8 µLtimate buster 1 is a protein that in humans is encoded by the NUB1 gene. This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants.
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分子量
- 75 kDa
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基因ID
- 51667
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UniProt
- Q9Y5A7
抗原
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