PHTF1 抗体 (AA 188-430)
Quick Overview for PHTF1 抗体 (AA 188-430) (ABIN7600390)
抗原
See all PHTF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 188-430
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原理
- Anti-PHTF1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PHTF1 Antibody Picoband® (ABIN7600390). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PHTF1 recombinant protein (Position: I188-Q430).
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亚型
- IgG
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anti-Putative Homeodomain Transcription Factor 1 (PHTF1) (AA 248-347) antibody
PHTF1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Putative Homeodomain Transcription Factor 1 (PHTF1) (AA 1-637) antibodyPHTF1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Putative Homeodomain Transcription Factor 1 (PHTF1) (AA 248-347) antibodyPHTF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B7 unconjugated
anti-Putative Homeodomain Transcription Factor 1 (PHTF1) antibodyPHTF1 适用: 人 DB 宿主: 小鼠 Monoclonal PHTF1R8 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Manuel, A., Beaupain, D., Romeo, P. H., Raich, N. Molecular characterization of a novel gene family (PHTF) conserved from Drosophila to mammals. Genomics 64: 216-220, 2000. 2. Raich, N., Mattei, M. G., Romeo, P. H., Beaupain, D. PHTF, a novel atypical homeobox gene on chromosome 1p13, is evolutionarily conserved. Genomics 59: 108-109, 1999. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PHTF1 (Putative Homeodomain Transcription Factor 1 (PHTF1))
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别名
- PHTF1
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背景
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Synonyms: Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FJ
Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Background: HTF1 (putative homeodomain transcription factor 1), also known as PHTF, is a potential transcription regulator. It is a ubiquitously expressed integral, multipass membrane protein with predominant expression in testis. PHTF1 is associated with the ER (endoplasmic reticulum) and contains one bHLH (basic helix-loop-helix) domain. It is present in the cell during meiosis and spermiogenesis but, by the end of spermiogenesis, is released from the mature cell within the residual bodies. This implies that PHTF1 may play a role in the spermatozoa maturation process. In addition, PHTF1 is believed to interact with FEM1B and may be responsible for recruiting FEM1B to the surface of the ER membrane. This suggests that PHTF1 acts as a sequestering or anchoring protein for FEM1B. Two PHTF1 isoforms exist due to alternate splicing events. Isoform 2 is the shorter form and lacks the amino acid residues 648 to 762.
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分子量
- 100 kDa
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基因ID
- 10745
抗原
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