POLR1B 抗体 (AA 18-1135)
Quick Overview for POLR1B 抗体 (AA 18-1135) (ABIN7600313)
抗原
See all POLR1B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 18-1135
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原理
- Anti-POLR1B Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-POLR1B Antibody Picoband® (ABIN7600313). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human POLR1B recombinant protein (Position: H18-V1135).
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亚型
- IgG
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anti-Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B) (AA 963-1072) antibody
POLR1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4H6 unconjugated
anti-Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B) (Internal Region) antibodyPOLR1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B) (AA 89-117), (N-Term) antibodyPOLR1B 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB31123 unconjugated
anti-Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B) (AA 88-117), (N-Term) antibodyPOLR1B 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B) (C-Term) antibodyPOLR1B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B) (AA 963-1072) antibodyPOLR1B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B) (AA 1007-1056) antibodyPOLR1B 适用: 人, 小鼠, Pig, 兔, 斑马鱼, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B) (AA 670-900) antibodyPOLR1B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Sanchez, E., Laplace-Builhe, B., Mau-Them, F. T., Richard, E., Goldenberg, A., Toler, T. L., Guignard, T., Gatinois, V., Vincent, M., Blanchet, C., Boland, A., Bihoreau, M. T., and 16 others. POLR1B and neural crest cell anomalies in Treacher Collins syndrome type 4. Genet. Med. 22: 547-556, 2020. 2. Seither, P., Grummt, I. Molecular cloning of RPA2, the gene encoding the second largest subunit of mouse RNA polymerase I. Genomics 37: 135-139, 1996. 3. Stumpf, A. M. Personal Communication. Baltimore, Md. 07/06/2020. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))
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别名
- POLR1B
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背景
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Synonyms: Solute carrier family 2, facilitated glucose transporter member 6, Glucose transporter type 6, GLUT-6, Glucose transporter type 9, GLUT-9, SLC2A6, GLUT9
Tissue Specificity: Highly expressed in brain, spleen and peripheral blood leukocytes.
Background: DNA-ed RNA polymerase I subunit RPA2 is an enzyme that in humans is encoded by the POLR1B gene. Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2.
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分子量
- 128 kDa
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基因ID
- 84172
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UniProt
- Q9H9Y6
抗原
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