PPM1F 抗体 (AA 17-454)
Quick Overview for PPM1F 抗体 (AA 17-454) (ABIN7600269)
抗原
See all PPM1F 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 17-454
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原理
- Anti-PPM1F Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PPM1F Antibody Picoband® (ABIN7600269). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PPM1F recombinant protein (Position: E17-S454).
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亚型
- IgG
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anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-76) antibody
PPM1F 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-100) antibodyPPM1F 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-100) antibodyPPM1F 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1G10 unconjugated
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) antibodyPPM1F 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-100) antibodyPPM1F 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) antibodyPPM1F 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 11-40), (N-Term) antibodyPPM1F 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB5923 unconjugated
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-190) antibodyPPM1F 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 275-303) antibody (HRP)PPM1F 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
anti-Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 275-303) antibody (PE)PPM1F 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal PE
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 4/30/2021. 2. Ishida, A., Sueyoshi, N., Kameshita, I. Functions and dysfunctions of Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) and CaMKP-N/PPM1E. Arch. Biochem. Biophys. 640: 83-92, 2018. 3. Kim, P. R., Koon, Y. L., Lee, R. T. C., Azizan, F., Koh, D. H. Z., Chiam, K.-H., Koh, C.-G. Phosphatase POPX2 interferes with cell cycle by interacting with Chk1. Cell Cycle 19: 405-418, 2020. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))
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别名
- PPM1F
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背景
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Synonyms: Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27
Tissue Specificity: Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.
Background: Protein phosphatase 1F is an enzyme that in humans is encoded by the PPM1F gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
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分子量
- 54 kDa
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基因ID
- 9647
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UniProt
- P49593
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途径
- Positive Regulation of Endopeptidase Activity
抗原
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