HPRT1 抗体 (AA 17-186)
Quick Overview for HPRT1 抗体 (AA 17-186) (ABIN7600250)
抗原
See all HPRT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 17-186
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原理
- Anti-HPRT1 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-HPRT1 Antibody Picoband® (ABIN7600250). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human HPRT1 recombinant protein (Position: Y17-K186).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ansari MY, Dikhit MR, Sahoo GC, Das P (Apr 2012). "Comparative modeling of HGPRT enzyme of L. donovani and binding affinities of different analogs of GMP". International Journal of Biological Macromolecules50 (3): 637-49. 2. Finette BA, Kendall H, Vacek PM (Aug 2002). "Mutational spectral analysis at the HPRT locus in healthy children". Mutation Research 505 (1-2): 27-41. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))
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别名
- HPRT1
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背景
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Synonyms: Thrombospondin-1,THBS1,TSP, TSP1,
Tissue Specificity: Expressed in natural killer (NK) cells, CD8 (+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs).
Background: Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
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分子量
- 25 kDa
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基因ID
- 3251
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UniProt
- P00492
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途径
- Ribonucleoside Biosynthetic Process
抗原
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