UBE2O 抗体 (AA 150-1283)
Quick Overview for UBE2O 抗体 (AA 150-1283) (ABIN7600121)
抗原
See all UBE2O 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 150-1283
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原理
- Anti-UBE2O Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-UBE2O Antibody Picoband® (ABIN7600121). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human UBE2O recombinant protein (Position: R150-A1283).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mashtalir, N., Daou, S., Barbour, H., Sen, N. N., Gagnon, J., Hammond-Martel, I., Dar, H. H., Therrien, M., Affar, E. B. Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O. Molec. Cell 54: 392-406, 2014. 2. Nagase, T., Kikuno, R., Hattori, A., Kondo, Y., Okumura, K., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 347-355, 2000. 3. Nguyen, A. T., Prado, M. A., Schmidt, P. J., Sendamarai, A. K., Wilson-Grady, J. T., Min, M., Campagna, D. R., Tian, G., Shi, Y., Dederer, V., Kawan, M., Kuehnle, N., Paulo, J. A., Yao, Y., Weiss, M. J., Justice, M. J., Gygi, S. P., Fleming, M. D., Finley, D. UBE2O remodels the proteome during terminal erythroid differentiation. Science 357: eaan0218, 2017. Note: Electronic Article. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))
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别名
- UBE2O
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背景
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Synonyms: Homer protein homolog 3,Homer-3,HOMER3,
Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Background: Ubiquitin-conjugating enzyme E2 O is a protein that in humans is encoded by the UBE2O gene. Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway, protein ubiquitination, and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.
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分子量
- 200 kDa
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基因ID
- 63893
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UniProt
- Q9C0C9
抗原
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