CA9 抗体 (AA 146-414)
Quick Overview for CA9 抗体 (AA 146-414) (ABIN7600076)
抗原
See all CA9 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 146-414
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原理
- Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband® (ABIN7600076). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Carbonic Anhydrase 9/CA9 recombinant protein (Position: D146-D414).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Hewett-Emmett, D., Tashian, R. E. Functional diversity, conservation, and convergence in the evolution of the alpha-, beta- and gamma- carbonic anhydrase gene families. Molec. Phylogenet. Evol. 5: 50-77, 1996. 2. Ivanov, S. V., Kuzmin, I., Wei, M.-H., Pack, S., Geil, L., Johnson, B. E., Stanbridge, E. J., Lerman, M. I. Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes. Proc. Nat. Acad. Sci. 95: 12596-12601, 1998. 3. Nakagawa, Y., Uemura, H., Hirao, Y., Yoshida, K., Saga, S., Yoshikawa, K. Radiation hybrid mapping of the human MN/CA9 locus to chromosome band 9p12-p13. Genomics 53: 118-119, 1998. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CA9 (Carbonic Anhydrase IX (CA9))
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别名
- CA9
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背景
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Synonyms: Myosin-binding protein C, cardiac-type, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC3
Tissue Specificity: Red blood cells.
Background: Carbonic anhydrase IX (CA9/CA IX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
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分子量
- 54 kDa
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基因ID
- 768
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UniProt
- Q16790
抗原
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