P4HB 抗体 (AA 146-363)
Quick Overview for P4HB 抗体 (AA 146-363) (ABIN7600075)
抗原
See all P4HB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 146-363
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原理
- Anti-P4HB Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-P4HB Antibody Picoband® (ABIN7600075). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human P4HB recombinant protein (Position: A146-D363).
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亚型
- IgG
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anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 1-508) antibody
P4HB 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) antibodyP4HB 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) antibodyP4HB 适用: 人 WB, IF, ICC, IHC (p), IEM 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (N-Term) antibodyP4HB 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 329-508) antibodyP4HB 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 1-508) antibodyP4HB 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 409-509) antibodyP4HB 适用: 大鼠 WB, IHC, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (C-Term) antibodyP4HB 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, Pig, 兔, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 499-509) antibodyP4HB 适用: 人, 小鼠, 大鼠, Hamster, Cow, 犬, 猴, 豚鼠, Pig, 绵羊, 兔, 非洲爪蟾, 小鸡 WB, IHC, ICC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 409-509) antibody (HRP)P4HB 适用: 大鼠 WB, IHC, IP, IF, ICC 宿主: 兔 Polyclonal HRP
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cheng, S. Y., Gong, Q. H., Parkinson, C., Robinson, E. A., Appella, E., Merlino, G. T., Pastan, I. The nucleotide sequence of a human cellular thyroid hormone-binding protein present in endoplasmic reticulum. J. Biol. Chem. 262: 11221-11227, 1987. 2. Koivu, J., Myllyla, R., Halaakoski, T., Pihlajaniemi, T., Tasanen, K., Kivirikko, K. I. A single polypeptide acts both as the beta subunit of prolyl 4-hydroxylase and as a protein disulfide-isomerase. J. Biol. Chem. 262: 6447-6449, 1987. 3. Mezghrani, A., Fassio, A., Benham, A., Simmen, T., Braakman, I., Sitia, R. Manipulation of oxidative protein folding and PDI redox state in mammalian cells. EMBO J. 20: 6288-6296, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
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别名
- P4HB
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背景
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Synonyms: Acid-sensing ion channel 1, ASIC1,Amiloride-sensitive cation channel 2, neuronal, Brain sodium channel 2, BNaC2, ASIC1, ACCN2, BNAC2
Tissue Specificity: Expressed in most or all neurons.
Background: Protein disulfide-isomerase, also known as the beta-subunit of prolyl 4-hydroxylase (P4HB), is an enzyme that in humans encoded by the P4HB gene. This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
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分子量
- 57 kDa
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基因ID
- 5034
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UniProt
- P07237
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途径
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
抗原
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