RCC1 抗体 (AA 14-421)
Quick Overview for RCC1 抗体 (AA 14-421) (ABIN7600024)
抗原
See all RCC1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 14-421
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原理
- Anti-RCC1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-RCC1 Antibody Picoband® (ABIN7600024). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RCC1 recombinant protein (Position: A14-S421).
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亚型
- IgG
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anti-Regulator of Chromosome Condensation 1 (RCC1) (AA 1-240) antibody
RCC1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) antibodyRCC1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) (AA 312-421) antibodyRCC1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F1 unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) (AA 312-421) antibodyRCC1 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1C1 unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) antibodyRCC1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) (AA 1-421) antibodyRCC1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) (AA 301-350) antibodyRCC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) (AA 1-421) antibodyRCC1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) antibodyRCC1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Regulator of Chromosome Condensation 1 (RCC1) antibodyRCC1 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 332CT2-1-1 unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bischoff, F. R., Maier, G., Tilz, G., Ponstingl, H. A 47- kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation. Proc. Nat. Acad. Sci. 87: 8617-8621, 1990. 2. Carazo-Salas, R. E., Guarguaglini, G., Gruss, O. J., Segref, A., Karsenti, E., Mattaj, I. W. Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation. Nature 400: 178-181, 1999. 3. Chen, T., Muratore, T. L., Schaner-Tooley, C. E., Shabanowitz, J., Hunt, D. F., Macara, I. G. N-terminal alpha-methylation of RCC1 is necessary for stable chromatin association and normal mitosis. Nature Cell Biol. 9: 596-603, 2007. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RCC1 (Regulator of Chromosome Condensation 1 (RCC1))
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别名
- RCC1
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背景
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Synonyms: Short transient receptor potential channel 5, TrpC5, Transient receptor protein 5, TRP-5, hTRP-5, hTRP5, TRPC5, TRP5
Tissue Specificity: Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.
Background: CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.
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分子量
- 45 kDa
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基因ID
- 1104
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UniProt
- P18754
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途径
- Chromatin Binding, Protein targeting to Nucleus
抗原
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