STIL 抗体 (AA 13-1287)
Quick Overview for STIL 抗体 (AA 13-1287) (ABIN7599923)
抗原
See all STIL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 13-1287
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原理
- Anti-STIL/SIL Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-STIL/SIL Antibody Picoband® (ABIN7599923). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human STIL/SIL recombinant protein (Position: N13-F1287).
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亚型
- IgG
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anti-SCL/TAL1 Interrupting Locus (STIL) (AA 388-637) antibody
STIL 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SCL/TAL1 Interrupting Locus (STIL) antibodySTIL 适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SCL/TAL1 Interrupting Locus (STIL) (AA 866-900), (C-Term) antibodySTIL 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB53223 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aplan, P. D., Jones, C. A., Chervinsky, D. S., Zhao, X., Ellsworth, M., Wu, C., McGuire, E. A., Gross, K. W. An scl gene product lacking the transactivation domain induces bony abnormalities and cooperates with LMO1 to generate T-cell malignancies in transgenic mice. EMBO J. 16: 2408-2419, 1997. 2. Aplan, P. D., Lombardi, D. P., Ginsberg, A. M., Cossman, J., Bertness, V. L., Kirsch, I. L. Disruption of the human SCL locus by 'illegitimate' V-(D)-J recombinase activity. Science 250: 1426-1429, 1990. 3. Aplan, P. D., Lombardi, D. P., Reaman, G. H., Sather, H. N., Hammond, G. D., Kirsch, I. R. Involvement of the putative hematopoietic transcription factor SCL in T-cell acute lymphoblastic leukemia. Blood 79: 1327-1333, 1992. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- STIL (SCL/TAL1 Interrupting Locus (STIL))
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别名
- STIL
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背景
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Synonyms: Prolactin regulatory element-binding protein,Mammalian guanine nucleotide exchange factor mSec12,PREB,SEC12,
Tissue Specificity: Ubiquitous. .
Background: SCL-interrupting locus protein is a protein that in humans is encoded by the STIL gene. This gene encodes a cytoplasmic protein implicated in regulation of the mitotic spindle checkpoint, a regulatory pathway that monitors chromosome segregation during cell division to ensure the proper distribution of chromosomes to daughter cells. The protein is phosphorylated in mitosis and in response to activation of the spindle checkpoint, and disappears when cells transition to G1 phase. It interacts with a mitotic regulator, and its expression is required to efficiently activate the spindle checkpoint. It is proposed to regulate Cdc2 kinase activity during spindle checkpoint arrest. Chromosomal deletions that fuse this gene and the adjacent locus commonly occur in T cell leukemias, and are thought to arise through illegitimate V- (D)-J recombination events. Multiple transcript variants encoding different isoforms have been found for this gene.
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分子量
- 170 kDa
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基因ID
- 6491
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UniProt
- Q15468
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途径
- Tube Formation
抗原
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