E2F5 抗体 (AA 121-346)
Quick Overview for E2F5 抗体 (AA 121-346) (ABIN7599838)
抗原
See all E2F5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 121-346
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原理
- Anti-E2F5 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-E2F5 Antibody Picoband® (ABIN7599838). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human E2F5 recombinant protein (Position: C121-Y346).
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亚型
- IgG
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anti-E2F5 (E2F5) (AA 101-200) antibody
E2F5 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (AA 66-93), (N-Term) antibodyE2F5 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23726 unconjugated
anti-E2F5 (E2F5) antibodyE2F5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (AA 193-298) antibodyE2F5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) antibodyE2F5 适用: 人 DB, ICC 宿主: 小鼠 Monoclonal 220D1a unconjugated
anti-E2F5 (E2F5) (AA 134-183) antibodyE2F5 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, 斑马鱼, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (AA 197-346) antibodyE2F5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (N-Term) antibodyE2F5 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, C.-R., Kang, Y., Siegel, P. M., Massague, J. E2F4/5 and p107 as Smad cofactors linking the TGF-beta receptor to c-myc repression. Cell 110: 19-32, 2002. 2. Gaubatz, S., Lindeman, G. J., Ishida, S., Jakoi, L., Nevins, J. R., Livingston, D. M., Rempel, R. E. E2F4 and E2F5 play an essential role in pocket protein-mediated G1 control. Molec. Cell 6: 729-735, 2000. 3. Gross, M. B. Personal Communication. Baltimore, Md. 7/28/2014. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- E2F5
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别名
- E2F5
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背景
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Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA
Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).
Background: Transcription factor E2F5 is a protein that in humans is encoded by the E2F5 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.
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分子量
- 45 kDa
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基因ID
- 1875
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UniProt
- Q15329
抗原
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