METTL14 抗体 (AA 12-350)
Quick Overview for METTL14 抗体 (AA 12-350) (ABIN7599817)
抗原
See all METTL14 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 12-350
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原理
- Anti-METTL14 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-METTL14 Antibody Picoband® (ABIN7599817). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human METTL14 recombinant protein (Position: Q12-D350).
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亚型
- IgG
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anti-Methyltransferase Like 14 (METTL14) (AA 1-210) antibody
METTL14 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 14 (METTL14) (AA 2-36) antibodyMETTL14 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB55130 unconjugated
anti-Methyltransferase Like 14 (METTL14) (AA 1-456) antibodyMETTL14 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Methyltransferase Like 14 (METTL14) (AA 21-120) antibodyMETTL14 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 14 (METTL14) (AA 35-84) antibodyMETTL14 适用: 人, 小鼠, Cow, 马, 猴, Pig, 兔, 大鼠, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/4/2015. 2. Liu, J., Yue, Y., Han, D., Wang, X., Fu, Y., Zhang, L., Jia, G., Yu, M., Lu, Z., Deng, X., Dai, Q., Chen, W., He, C. A METTL3-METTL14 complex mediates mammalian nuclear RNA N(6)-adenosine methylation. Nature Chem. Biol. 10: 93-95, 2014. 3. Nagase, T., Kikuno, R., Nakayama, M., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 273-281, 2000. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- METTL14 (Methyltransferase Like 14 (METTL14))
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别名
- METTL14
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背景
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Synonyms: Mucin-20, MUC-20, MUC20, KIAA1359, UNQ2782/PRO7170
Tissue Specificity: Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis.
Background: Methyltransferase like 14 is a protein that in humans is encoded by the METTL14 gene. METTL14 is part of a methyltransferase complex required for the common N6-methyladenosine (m6A) base modification that is enriched near stop codons and in long exons of mRNAs.
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分子量
- 65 kDa
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基因ID
- 57721
抗原
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