RAP1GAP 抗体 (AA 11-661)
Quick Overview for RAP1GAP 抗体 (AA 11-661) (ABIN7599729)
抗原
See all RAP1GAP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 11-661
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原理
- Anti-RAP1GAP Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-RAP1GAP Antibody Picoband® (ABIN7599729). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RAP1GAP recombinant protein (Position: D11-L661).
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亚型
- IgG
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anti-RAP1 GTPase Activating Protein (RAP1GAP) (Internal Region) antibody
RAP1GAP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) (AA 412-660) antibodyRAP1GAP 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2G7B6 unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) (AA 412-678) antibodyRAP1GAP 适用: 人 宿主: 小鼠 Monoclonal unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) (C-Term) antibodyRAP1GAP 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) (C-Term) antibodyRAP1GAP 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) antibodyRAP1GAP 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) (AA 451-550) antibodyRAP1GAP 适用: 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) (C-Term) antibodyRAP1GAP 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) (AA 105-128) antibodyRAP1GAP 适用: 人 WB 宿主: 兔 Polyclonal RB53561 unconjugated
anti-RAP1 GTPase Activating Protein (RAP1GAP) (C-Term) antibodyRAP1GAP 适用: 人, 小鼠, Cow, 豚鼠, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Daumke, O., Weyand, M., Chakrabarti, P. P., Vetter, I. R., Wittinghofer, A. The GTPase-activating protein Rap1GAP uses a catalytic asparagine. Nature 429: 197-201, 2004. 2. Rubinfeld, B., Munemitsu, S., Clark, R., Conroy, L., Watt, K., Crosier, W. J., McCormick, F., Polakis, P. Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21-rap1. Cell 65: 1033-1042, 1991. 3. Weiss, J., Rubinfeld, B., Polakis, P. G., McCormick, F., Cavenee, W. K., Arden, K. C. The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-p35. Cytogenet. Cell Genet. 66: 18-21, 1994. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- RAP1GAP (RAP1 GTPase Activating Protein (RAP1GAP))
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别名
- RAP1GAP
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背景
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Synonyms: Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor, NHEJ1, XLF
Tissue Specificity: Widely expressed.
Background: Rap1 GTPase-activating protein 1 is an enzyme that in humans is encoded by the RAP1GAP gene. This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.
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分子量
- 95 kDa
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基因ID
- 5909
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UniProt
- P47736
抗原
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