EDC4 抗体 (AA 1082-1388)
Quick Overview for EDC4 抗体 (AA 1082-1388) (ABIN7599694)
抗原
See all EDC4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1082-1388
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原理
- Anti-EDC4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EDC4 Antibody Picoband® (ABIN7599694). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EDC4 recombinant protein (Position: K1082-L1388).
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亚型
- IgG
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anti-Enhancer of mRNA Decapping 4 (EDC4) (AA 753-952) antibody
EDC4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of mRNA Decapping 4 (EDC4) (AA 1302-1401) antibodyEDC4 适用: 人 ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2E2 unconjugated
anti-Enhancer of mRNA Decapping 4 (EDC4) (AA 1-1401) antibodyEDC4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Enhancer of mRNA Decapping 4 (EDC4) (C-Term) antibodyEDC4 适用: 人, 小鼠, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of mRNA Decapping 4 (EDC4) (AA 74-123) antibodyEDC4 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, Cow, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of mRNA Decapping 4 (EDC4) (AA 800-1100) antibodyEDC4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of mRNA Decapping 4 (EDC4) (N-Term) antibodyEDC4 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of mRNA Decapping 4 (EDC4) (N-Term) antibodyEDC4 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, Cow, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bloch, D. B., Rabkina, D., Quertermous, T., Bloch, K. D. The immunoreactive region in a novel autoantigen contains a nuclear localization sequence. Clin. Immun. Immunopath. 72: 380-389, 1994. 2. Fenger-Gron, M., Fillman, C., Norrild, B., Lykke-Andersen, J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Molec. Cell 20: 905-915, 2005. 3. Garcia-Lozano, J. R., Gonzalez-Escribano, M. F., Wichmann, I., Nunez-Roldan, A. Cytoplasmic detection of a novel protein containing a nuclear localization sequence by human autoantibodies. Clin. Exp. Immun. 107: 501-506, 1997. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EDC4 (Enhancer of mRNA Decapping 4 (EDC4))
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别名
- EDC4
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背景
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Synonyms: Protein Dok-7, Downstream of tyrosine kinase 7, DOK7, C4orf25,
Tissue Specificity: Preferentially expressed in skeletal muscle and heart. Present in thigh muscle, diaphragm and heart but not in the liver or spleen .
Background: Enhancer of mRNA-decapping protein 4 is a protein that in humans is encoded by the EDC4 gene. Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjgren's syndrome patient later diagnosed with primary biliary cirrhosis. EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation. Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182. Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression.
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分子量
- 152 kDa
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基因ID
- 23644
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UniProt
- Q6P2E9
抗原
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