Aurora A 抗体 (AA 1-97)
Quick Overview for Aurora A 抗体 (AA 1-97) (ABIN7599581)
抗原
See all Aurora A (AURKA) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-97
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原理
- Anti-Aurora A/AURKA Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Aurora A/AURKA Antibody Picoband® (ABIN7599581). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Aurora A/AURKA recombinant protein (Position: M1-K97).
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亚型
- IgG
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anti-Aurora Kinase A (AURKA) (AA 1-110) antibody
AURKA 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5F8 unconjugated
anti-Aurora Kinase A (AURKA) (C-Term) antibodyAURKA 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase A (AURKA) (AA 268-404) antibodyAURKA 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 2D8A4 unconjugated
anti-Aurora Kinase A (AURKA) (AA 364-392), (C-Term) antibodyAURKA 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB6834 unconjugated
anti-Aurora Kinase A (AURKA) antibodyAURKA 适用: 人, 大鼠, 猴 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-Aurora Kinase A (AURKA) (pThr288) antibodyAURKA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase A (AURKA) (AA 268-404) antibodyAURKA 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 4H5A8 unconjugated
anti-Aurora Kinase A (AURKA) antibodyAURKA 适用: 人, 大鼠, 猴 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 4G10 unconjugated
anti-Aurora Kinase A (AURKA) (AA 364-392) antibodyAURKA 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase A (AURKA) (AA 1-131) antibodyAURKA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bischoff, J. R., Plowman, G. D. The Aurora/lpl 1p kinase family: regulators of chromosome segregation and cytokinesis. Trends Cell Biol. 9: 454-459, 1999. 2. Hutterer, A., Berdnik, D., Wirtz-Peitz, F., Zigman, M., Schleiffer, A., Knoblich, J. A. Mitotic activation of the kinase Aurora-A requires its binding partner Bora. Dev. Cell 11: 147-157, 2006. 3. Seki, A., Coppinger, J. A., Jang, C.-Y., Yates, J. R., III, Fang, G. Bora and the kinase Aurora A cooperatively activate the kinase Plk1 and control mitotic entry. Science 320: 1655-1658, 2008. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Aurora A (AURKA) (Aurora Kinase A (AURKA))
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别名
- AURKA
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背景
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Synonyms: Probable ATP-dependent RNA helicase DDX58,3.6.4.13,DEAD box protein 58,RIG-I-like receptor 1,RLR-1,Retinoic acid-inducible gene 1 protein,RIG-1,Retinoic acid-inducible gene I protein,RIG-I,DDX58,
Tissue Specificity: Present in vascular smooth cells (at protein level). .
Background: AURKA(aurora kinase A), also called ARK1, AurA, AIK , AURORA2 ,BTAK, PPP1R47, STK7, STK15,STK6, is a mitotic centrosomal protein kinase. The main role of AURKA in tumor development is in controlling chromosome segregation during mitosis. Aurora A is a member of a family of mitotic serine/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of AURKA occurs during the G2/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Activation of AURKA was independently sufficient to induce rapid ciliary resorption, and AURKA acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of AURKA and HDAC6 reduced regulated disassembly of cilia.
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分子量
- 50 kDa
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基因ID
- 6790
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UniProt
- O14965
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途径
- Cell Division Cycle, Asymmetric Protein Localization
抗原
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