N4BP1 抗体 (AA 1-885)
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- 抗原 See all N4BP1 抗体
- N4BP1 (NEDD4 Binding Protein 1 (N4BP1))
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抗原表位
- AA 1-885
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This N4BP1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 原理
- Anti-N4BP1 Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-N4BP1 Antibody Picoband® (ABIN7599569). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human N4BP1 recombinant protein (Position: M1-K885).
- 亚型
- IgG
- Top Product
- Discover our top product N4BP1 Primary Antibody
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anti-NEDD4 Binding Protein 1 (N4BP1) (Internal Region) antibody
N4BP1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NEDD4 Binding Protein 1 (N4BP1) (AA 190-590) antibodyN4BP1 适用: 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-NEDD4 Binding Protein 1 (N4BP1) (AA 1-896) antibodyN4BP1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NEDD4 Binding Protein 1 (N4BP1) (Internal Region) antibodyN4BP1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gitlin, A. D., Heger, K., Schubert, A. F., Reja, R., Yan, D., Pham, V. C., Suto, E., Zhang, J., Kwon, Y. C., Freund, E. C., Kang, J., Pham, A., and 13 others. Integration of innate immune signalling by caspase-8 cleavage of N4BP1. Nature 587: 275-280, 2020. 2. Gross, M. B. Personal Communication. Baltimore, Md. 12/23/2020. 3. Murillas, R., Simms, K. S., Hatakeyama, S., Weissman, A. M., Kuehn, M. R. Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. J. Biol. Chem. 277: 2897-2907, 2002. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- 抗原
- N4BP1 (NEDD4 Binding Protein 1 (N4BP1))
- 别名
- N4BP1 (N4BP1 产品)
- 背景
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Synonyms: Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FJ
Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Background: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73α, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73α for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.
- 分子量
- 110 kDa
- 基因ID
- 9683
- UniProt
- O75113
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