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PEPCK 抗体 (AA 1-640)

This anti-PEPCK antibody is a 小鼠 单克隆 antibody detecting PEPCK in WB, IHC, IF, ICC 和 FACS. Suitable for 人, 小鼠, 大鼠 和 猴.
产品编号 ABIN7599509
发货至: 中国

Quick Overview for PEPCK 抗体 (AA 1-640) (ABIN7599509)

抗原

See all PEPCK 抗体
PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

适用

人, 小鼠, 大鼠, 猴

宿主

  • 60
  • 8
  • 1
小鼠

克隆类型

  • 53
  • 16
单克隆

标记

  • 33
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PEPCK antibody is un-conjugated

应用范围

  • 52
  • 25
  • 19
  • 14
  • 14
  • 10
  • 9
  • 8
  • 6
  • 5
  • 4
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

克隆位点

3F7
  • 抗原表位

    • 15
    • 8
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-640

    原理

    Anti-PCK2 Antibody Picoband® (monoclonal, 3F7)

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-PCK2 Antibody Picoband® (monoclonal, 3F7) (ABIN7599509). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human PCK2 recombinant protein (Position: M1-M640).

    亚型

    IgG2b
  • 应用备注

    Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Modaressi, S., Brechtel, K., Christ, B., Jungermann, K. Human mitochondrial phosphoenolpyruvate carboxykinase 2 gene: structure, chromosomal localization and tissue-specific expression Biochem. J. 333: 359-366, 1998. 2. Modaressi, S., Christ, B., Bratke, J., Zahn, S., Heise, T., Jungermann, K. Molecular cloning, sequencing and expression of the cDNA of the mitochondrial form of phosphoenolpyruvate carboxykinase from human liver. Biochem. J. 315: 807-814, 1996.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

    别名

    PCK2

    背景

    Synonyms: Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2

    Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

    Background: Phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2, PEPCK-M), is an isozyme of phosphoenolpyruvate carboxykinase (PCK, PEPCK) that in humans is encoded by the PCK2 gene. It is mapped to 14q11.2-q12. This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

    分子量

    71 kDa

    基因ID

    5106

    UniProt

    Q16822

    途径

    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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