PEPCK 抗体 (AA 1-640)
Quick Overview for PEPCK 抗体 (AA 1-640) (ABIN7599508)
抗原
See all PEPCK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-640
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原理
- Anti-PCK2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PCK2 Antibody Picoband® (ABIN7599508). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PCK2 recombinant protein (Position: M1-M640).
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亚型
- IgG
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anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (Internal Region) antibody
Verified PEPCK 适用: 人, 小鼠 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) antibodyPEPCK 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 44-175) antibodyPEPCK 适用: 人, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3D3D9 unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibodyPEPCK 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 33-198) antibodyPEPCK 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) antibodyPEPCK 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibodyPEPCK 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C9 unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-280) antibodyPEPCK 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 24-54), (N-Term) antibodyPEPCK 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB3640 unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibodyPEPCK 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Modaressi, S., Brechtel, K., Christ, B., Jungermann, K. Human mitochondrial phosphoenolpyruvate carboxykinase 2 gene: structure, chromosomal localization and tissue-specific expression Biochem. J. 333: 359-366, 1998. 2. Modaressi, S., Christ, B., Bratke, J., Zahn, S., Heise, T., Jungermann, K. Molecular cloning, sequencing and expression of the cDNA of the mitochondrial form of phosphoenolpyruvate carboxykinase from human liver. Biochem. J. 315: 807-814, 1996. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
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别名
- PCK2
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背景
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Synonyms: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, PCK2, PEPCK2
Tissue Specificity: Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.
Background: Phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2, PEPCK-M), is an isozyme of phosphoenolpyruvate carboxykinase (PCK, PEPCK) that in humans is encoded by the PCK2 gene. It is mapped to 14q11.2-q12. This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
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分子量
- 71 kDa
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基因ID
- 5106
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UniProt
- Q16822
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途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
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