EIF4B 抗体 (AA 1-611)
Quick Overview for EIF4B 抗体 (AA 1-611) (ABIN7599496)
抗原
See all EIF4B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-611
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原理
- Anti-EIF4B Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EIF4B Antibody Picoband® (ABIN7599496). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EIF4B recombinant protein (Position: M1-E611).
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody
EIF4B 适用: 人, 小鼠 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibodyEIF4B 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 352-611) antibodyEIF4B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibodyEIF4B 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 546-575), (C-Term) antibodyEIF4B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB11717 unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (C-Term) antibodyEIF4B 适用: 人, 小鼠, 大鼠, 马, Cow, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 353-382) antibodyEIF4B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibodyEIF4B 适用: 人, 小鼠 WB, IF, EIA 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 362-590) antibodyEIF4B 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 150-200) antibodyEIF4B 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Garcia-Garcia, C., Frieda, K. L., Feoktistova, K., Fraser, C. S., Block, S. M. Factor-dependent processivity in human eIF4A DEAD-box helicase. Science 348: 1486-1488, 2015. 2. Methot, N., Pause, A., Hershey, J. W., Sonenberg, N. The translation initiation factor eIF-4B contains an RNA-binding region that is distinct and independent from its ribonucleoprotein consensus sequence. Molec. Cell. Biol. 14: 2307-2316, 1994. 3. Methot, N., Song, M. S., Sonenberg, N. A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3. Molec. Cell. Biol. 16: 5328-5334, 1996. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))
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别名
- EIF4B
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背景
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Synonyms: Complement component C7, C7
Tissue Specificity: Widely expressed.
Background: Eukaryotic translation initiation factor 4B is a protein that in humans is encoded by the EIF4B gene. Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens, and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.
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分子量
- 80 kDa
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基因ID
- 1975
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UniProt
- P23588
抗原
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