TRIM25 抗体 (AA 1-610)
Quick Overview for TRIM25 抗体 (AA 1-610) (ABIN7599495)
抗原
See all TRIM25 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-610
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原理
- Anti-TRIM25/EFP Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-TRIM25/EFP Antibody Picoband® (ABIN7599495). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human TRIM25/EFP recombinant protein (Position: M1-A610).
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亚型
- IgG
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anti-Tripartite Motif Containing 25 (TRIM25) (AA 211-360) antibody
TRIM25 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 5B5B12 unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) (AA 100-400) antibodyTRIM25 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) (AA 211-310) antibodyTRIM25 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5C3 unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) (AA 81-272) antibodyTRIM25 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) (AA 1-630) antibodyTRIM25 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) (AA 1-630) antibodyTRIM25 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) antibodyTRIM25 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) (C-Term) antibodyTRIM25 适用: 人, 小鼠 WB, ELISA, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) (AA 243-491) antibodyTRIM25 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 25 (TRIM25) (AA 531-630) antibodyTRIM25 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gack, M. U., Shin, Y. C., Joo, C.-H., Urano, T., Liang, C., Sun, L., Takeuchi, O., Akira, S., Chen, Z., Inoue, S., Jung, J. U. TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. Nature 446: 916-920, 2007. 2. Inoue, S., Orimo, A., Hosoi, T., Kondo, S., Toyoshima, H., Kondo, T., Ikegami, A., Ouchi, Y., Orimo, H., Muramatsu, M. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Nat. Acad. Sci. 90: 11117-11121, 1993. 3. Manokaran, G., Finol, E., Wang, C., Gunaratne, J., Bahl, J., Ong, E. Z., Tan, H. C., Sessions, O. M., Ward, A. M., Gubler, D. J., Harris, E., Garcia-Blanco, M. A., Ooi, E. E. Dengue subgenomic RNA binds TRIM25 to inhibit interferon expression for epidemiological fitness. Science 350: 217-221, 2015. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- TRIM25 (Tripartite Motif Containing 25 (TRIM25))
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别名
- TRIM25
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背景
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Synonyms: E3 ubiquitin/ISG15 ligase TRIM25, Estrogen-responsive finger protein, RING finger protein 147, RING-type E3 ubiquitin transferase, RING-type E3 ubiquitin transferase TRIM25, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147
Tissue Specificity: Expressed in breast tumors (at protein level). Ubiquitous.
Background: Tripartite motif-containing protein 25 is a protein that in humans is encoded by the TRIM25 gene. It is mapped to 17q22. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
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分子量
- 71 kDa
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基因ID
- 7706
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UniProt
- Q14258
抗原
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