REC8 抗体 (AA 1-547)
Quick Overview for REC8 抗体 (AA 1-547) (ABIN7599473)
抗原
See all REC8 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1-547
-
原理
- Anti-REC8 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-REC8 Antibody Picoband® (ABIN7599473). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human REC8 recombinant protein (Position: M1-H547).
-
亚型
- IgG
-
-
anti-REC8 Homolog (Yeast) (REC8) (AA 1-270) antibody
REC8 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-REC8 Homolog (Yeast) (REC8) (pSer251) antibodyREC8 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-REC8 Homolog (Yeast) (REC8) (Internal Region) antibodyREC8 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-REC8 Homolog (Yeast) (REC8) (N-Term) antibodyREC8 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-REC8 Homolog (Yeast) (REC8) (AA 22-120) antibodyREC8 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-REC8 Homolog (Yeast) (REC8) (AA 1-110) antibodyREC8 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2G3 unconjugated
anti-REC8 Homolog (Yeast) (REC8) (AA 1-547) antibodyREC8 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-REC8 Homolog (Yeast) (REC8) (AA 72-121) antibodyREC8 适用: 人, Cow, 犬, 豚鼠, 马, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-REC8 Homolog (Yeast) (REC8) (AA 194-561) antibodyREC8 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-REC8 Homolog (Yeast) (REC8) (AA 194-222) antibodyREC8 适用: 人 WB 宿主: 兔 Polyclonal RB32542 unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brar, G. A., Kiburz, B. M., Zhang, Y., Kim, J.-E., White, F., Amon, A. Rec8 phosphorylation and recombination promote the step-wise loss of cohesins in meiosis. Nature 441: 532-536, 2006. 2. Parisi, S., McKay, M. J., Molnar, M., Thompson, M. A., van der Spek, P. J., van Drunen-Schoenmaker, E., Kanaar, R., Lehmann, E., Hoeijmakers, J. H. J., Kohli, J. Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans. Molec. Cell. Biol. 19: 3515-3528, 1999. 3. Xu, H., Beasley, M. D., Warren, W. D., van der Horst, G. T. J., McKay, M. J. Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis. Dev. Cell 8: 949-961, 2005. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- REC8 (REC8 Homolog (Yeast) (REC8))
-
别名
- REC8
-
背景
-
Synonyms: Meiotic recombination protein REC8 homolog, Cohesin Rec8p, REC8, REC8L1
Tissue Specificity: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa.
Background: Meiotic recombination protein REC8 homolog is a protein that in humans is encoded by the REC8 gene. It is mapped to 14q12. This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
-
分子量
- 63 kDa
-
基因ID
- 9985
-
UniProt
- O95072
抗原
-
