NOB1 抗体 (AA 1-389)
Quick Overview for NOB1 抗体 (AA 1-389) (ABIN7599344)
抗原
See all NOB1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1-389
-
原理
- Anti-NOB1 Antibody Picoband®
-
交叉反应 (详细)
- No cross-reactivity with other proteins.
-
产品特性
- Anti-NOB1 Antibody Picoband® (ABIN7599344). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human NOB1 recombinant protein (Position: M1-D389).
-
亚型
- IgG
-
-
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Ameismeier, M., Cheng, J., Berninghausen, O., Beckmann, R. Visualizing late states of human 40S ribosomal subunit maturation. Nature 558: 249-253, 2018. 2. Fatica, A., Oeffinger, M., Dlakic, M., Tollervey, D. Nob1p is required for cleavage of the 3-prime end of 18S rRNA. Molec. Cell. Biol. 23: 1798-1807, 2003. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 10/6/2010. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- NOB1 (RNA-Binding Protein NOB1 (NOB1))
-
别名
- NOB1
-
背景
-
Synonyms: Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2, LMO2, RBTN2, RBTNL1, RHOM2, TTG2
Background: RNA-binding protein NOB1 is a protein that in humans is encoded by the NOB1 gene. In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.
-
分子量
- 47 kDa
-
基因ID
- 28987
-
途径
- Protein targeting to Nucleus
抗原
-