NEK7 抗体 (AA 1-302)
Quick Overview for NEK7 抗体 (AA 1-302) (ABIN7599245)
抗原
See all NEK7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-302
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原理
- Anti-NEK7 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-NEK7 Antibody Picoband® (ABIN7599245). Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NEK7 recombinant protein (Position: M1-S302). Human NEK7 shares 97.7% and 98% amino acid (aa) sequence identity with mouse and rat NEK7, respectively.
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亚型
- IgG
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anti-NEK7 (NEK7) (N-Term) antibody
NEK7 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, Pig, 猴, Bat, 小鸡, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NEK7 (NEK7) antibodyNEK7 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NEK7 (NEK7) (Kinase Domain) antibodyNEK7 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NEK7 (NEK7) (Internal Region) antibodyVerified NEK7 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-NEK7 (NEK7) (AA 1-302) antibodyNEK7 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NEK7 (NEK7) (Internal Region) antibodyNEK7 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-NEK7 (NEK7) (AA 1-30), (N-Term) antibodyNEK7 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2967-2968 unconjugated
anti-NEK7 (NEK7) (AA 1-302) antibodyNEK7 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-NEK7 (NEK7) (AA 1-30) antibodyNEK7 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NEK7 (NEK7) (AA 274-286) antibodyVerified NEK7 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 2-4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. He, Y., Zeng, M. Y., Yang, D., Motro, B., Nunez, G. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 530: 354-357, 2016. 2. Kimura, M., Okano, Y. Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3. Cytogenet. Cell Genet. 94: 33-38, 2001. 3. Sharif, H., Wang, L., Wang, W. L., Magupalli, V. G., Andreeva, L., Qiao, Q., Hauenstein, A. V., Wu, Z., Nunez, G., Mao, Y., Wu, H. Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome. Nature 570: 338-343, 2019. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NEK7
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别名
- NEK7
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背景
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Synonyms: NEK7, Serine/threonine-protein kinase Nek7, EC 2.7.11.1, Never in mitosis A-related kinase 7, NimA-related protein kinase 7
Background: NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.
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分子量
- 35 kDa
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基因ID
- 140609
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途径
- Inflammasome
抗原
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