NUF2 抗体 (AA 1-297)
Quick Overview for NUF2 抗体 (AA 1-297) (ABIN7599236)
抗原
See all NUF2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-297
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原理
- Anti-NUF2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NUF2 Antibody Picoband® (ABIN7599236). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NUF2 recombinant protein (Position: M1-Q297).
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亚型
- IgG
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anti-NUF2, NDC80 Kinetochore Complex Component (NUF2) (AA 201-300) antibody
NUF2 适用: 人, 小鼠 ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-NUF2, NDC80 Kinetochore Complex Component (NUF2) (AA 165-464) antibodyNUF2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NUF2, NDC80 Kinetochore Complex Component (NUF2) antibodyNUF2 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-NUF2, NDC80 Kinetochore Complex Component (NUF2) (AA 1-464) antibodyNUF2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. DeLuca, J. G., Moree, B., Hickey, J. M., Kilmartin, J. V., Salmon, E. D. hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J. Cell Biol. 159: 549-555, 2002. 2. Liu, D., Ding, X., Du, J., Cai, X., Huang, Y., Ward, T., Shaw, A., Yang, Y., Hu, R., Jin, C., Yao, X. Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J. Biol. Chem. 282: 21415-21424, 2007. 3. Nabetani, A., Koujin, T., Tsutsumi, C., Haraguchi, T., Hiraoka, Y. A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint. Chromosoma 110: 322-334, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))
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别名
- NUF2
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背景
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Synonyms: S-arrestin, 48 kDa protein, Retinal S-antigen, S-AG, Rod photoreceptor arrestin, SAG,
Tissue Specificity: Retina and pineal gland.
Background: Kinetochore protein Nuf2 is a protein that in humans is encoded by the NUF2 gene. This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
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分子量
- 54 kDa
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基因ID
- 83540
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途径
- Maintenance of Protein Location
抗原
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