PNPT1 抗体 (AA 1-257)
Quick Overview for PNPT1 抗体 (AA 1-257) (ABIN7599172)
抗原
See all PNPT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-257
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原理
- Anti-PNPT1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PNPT1 Antibody Picoband® (ABIN7599172). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PNPT1 recombinant protein (Position: M1-Q257).
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亚型
- IgG
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anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (AA 1-783) antibody
PNPT1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (AA 489-538) antibodyPNPT1 适用: 人, 大鼠, 犬, 马, Cow, 豚鼠, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (AA 1-783) antibodyPNPT1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) antibodyPNPT1 适用: 人 WB, IP, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (N-Term) antibodyPNPT1 适用: 人 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (Middle Region) antibodyPNPT1 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) antibodyPNPT1 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (AA 551-650) antibodyPNPT1 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (C-Term) antibodyPNPT1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) (C-Term) antibodyPNPT1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Liu, X., Fu, R., Pan, Y., Meza-Sosa, K. F., Zhang, Z., Lieberman, J. PNPT1 release from mitochondria during apoptosis triggers decay of poly(A) RNAs. Cell 174: 187-201, 2018. 2. Vedrenne, V., Gowher, A., De Lonlay, P., Nitschke, P., Serre, V., Boddaert, N., Altuzarra, C., Mager-Heckel, A.-M., Chretien, F., Entelis, N., Munnich, A., Tarassov, I., Rotig, A. Mutation in PNPT1, which encodes a polyribonucleotide nucleotidyltransferase, impairs RNA import into mitochondria and causes respiratory-chain deficiency. Am. J. Hum. Genet. 91: 912-918, 2012. 3. von Ameln, S., Wang, G., Boulouiz, R., Rutherford, M. A., Smith, G. M., Li, Y., Pogoda, H.-M., Nurnberg, G., Stiller, B., Volk, A. E., Borck, G., Hong, J. S., and 12 others. A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss. Am. J. Hum. Genet. 91: 919-927, 2012. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
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别名
- PNPT1
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背景
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Synonyms: Prion-like protein doppel, PrPLP, Prion protein 2, PRND, DPL, UNQ1830/PRO3443
Tissue Specificity: Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level).
Background: The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7.
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分子量
- 86 kDa
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基因ID
- 87178
抗原
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