YWHAE 抗体 (AA 1-255)
Quick Overview for YWHAE 抗体 (AA 1-255) (ABIN7599165)
抗原
See all YWHAE 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-255
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原理
- Anti-YWHAE Antibody Picoband® (monoclonal, 3G11G2)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) (ABIN7599165). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human YWHAE recombinant protein (Position: M1-Q255).
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亚型
- IgG2b
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anti-14-3-3 epsilon (YWHAE) (AA 1-255) antibody
YWHAE 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) antibodyYWHAE 适用: 人 WB, IHC (p) 宿主: 兔 Monoclonal 2E2 unconjugated
anti-14-3-3 epsilon (YWHAE) antibodyYWHAE 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-YWHAE-1 unconjugated
anti-14-3-3 epsilon (YWHAE) (C-Term) antibodyYWHAE 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (C-Term) antibodyYWHAE 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 绵羊, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (Middle Region) antibodyYWHAE 适用: 小鼠, 斑马鱼, 豚鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) antibodyYWHAE 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (AA 151-255) antibodyYWHAE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (C-Term) antibodyYWHAE 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (C-Term) antibodyYWHAE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse, Rat
1. Bi, W., Sapir, T., Shchelochkov, O. A., Zhang, F., Withers, M. A., Hunter, J. V., Levy, T., Shinder, V., Peiffer, D. A., Gunderson, K. L., Nezarati, M. M., Shotts, V. A., and 13 others. Increased LIS1 expression affects human and mouse brain development. Nature Genet. 41: 168-177, 2009. 2. Cardoso, C., Leventer, R. J., Ward, H. L., Toyo-oka, K., Chung, J., Gross, A., Martin, C. L., Allanson, J., Pilz, D. T., Olney, A. H., Mutchinick, O. M., Hirotsune, S., Wynshaw-Boris, A., Dobyns, W. B., Ledbetter, D. H. Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3. Am. J. Hum. Genet. 72: 918-930, 2003. 3. Chong, S. S., Tanigami, A., Roschke, A. V., Ledbetter, D. H. 14-3-3-epsilon has no homology to LIS1 and lies telomeric to it on chromosome 17p13.3 outside the Miller-Dieker syndrome chromosome region. Genome Res. 6: 735-741, 1996. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- YWHAE (14-3-3 epsilon (YWHAE))
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别名
- YWHAE
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背景
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Synonyms: A32 antigen antibody, CD 146 antibody, CD146 antibody, CD146 antigen antibody, Cell surface glycoprotein MUC18 antibody, Cell surface glycoprotein P1H12 antibody, Gicerin antibody, MCAM antibody, Melanoma adhesion molecule antibody, Melanoma associated antigen A32 antibody, Melanoma associated antigen MUC18 antibody, Melanoma associated glycoprotein MUC18 antibody, Melanoma cell adhesion molecule antibody, Melanoma-associated antigen A32 antibody, Melanoma-associated antigen MUC18 antibody, MelCAM antibody, MUC 18 antibody, MUC18 antibody, MUC18_HUMAN antibody, S endo 1 antibody, S endo 1 endothelial associated antigen antibody, S-endo 1 endothelial-associated antigen antibody, Sendo 1 endothelial associated antigen antibody, Sendo1 antibody
Tissue Specificity: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
Background: 14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
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分子量
- 29 kDa
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基因ID
- 7531
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UniProt
- P62258
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途径
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
抗原
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