NIT2 抗体 (AA 1-249)
Quick Overview for NIT2 抗体 (AA 1-249) (ABIN7599155)
抗原
See all NIT2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-249
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原理
- Anti-NIT2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NIT2 Antibody Picoband® (ABIN7599155). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NIT2 recombinant protein (Position: M1-K249).
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亚型
- IgG
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anti-Nitrilase Family, Member 2 (NIT2) (AA 41-140) antibody
NIT2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nitrilase Family, Member 2 (NIT2) antibodyNIT2 适用: 人 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2B9 unconjugated
anti-Nitrilase Family, Member 2 (NIT2) (AA 1-276) antibodyNIT2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nitrilase Family, Member 2 (NIT2) (AA 72-121) antibodyNIT2 适用: 人, 小鼠, 犬, 豚鼠, 马, 兔, Hamster, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nitrilase Family, Member 2 (NIT2) (Middle Region) antibodyNIT2 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, Cow, Saccharomyces cerevisiae, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Asakura, M., Nakano, M., Hayashida, K., Fujii, H., Nakajima, M., Atsuda, K., Itoh, T., Fujiwara, R. Human nitrilase-like protein does not catalyze the hydrolysis of vildagliptin. Drug Metab. Pharmacokinet. 29: 463-469, 2014. 2. Chien, C.-H., Gao, Q.-Z., Cooper, A. J. L., Lyu, J.-H., Sheu, S.-Y. Structural insights into the catalytic active site and activity of human Nit2/omega-amidase: kinetic assay and molecular dynamics simulation. J. Biol. Chem. 287: 25715-25726, 2012. 3. Jaisson, S., Veiga-da-Cunha, M., Van Schaftingen, E. Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2. Biochimie 91: 1066-1071, 2009. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NIT2 (Nitrilase Family, Member 2 (NIT2))
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别名
- NIT2
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背景
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Synonyms: Troponin T, fast skeletal muscle, TnTf, Beta-TnTF, Fast skeletal muscle troponin T, fTnT, TNNT3
Tissue Specificity: In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.
Background: NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.
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分子量
- 33 kDa
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基因ID
- 56954
抗原
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