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NIT2 抗体 (AA 1-249)

This anti-NIT2 antibody is a 兔 多克隆 antibody detecting NIT2 in WB, ELISA, IHC 和 FACS. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN7599155
发货至: 中国

Quick Overview for NIT2 抗体 (AA 1-249) (ABIN7599155)

抗原

See all NIT2 抗体
NIT2 (Nitrilase Family, Member 2 (NIT2))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This NIT2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • 抗原表位

    • 7
    • 2
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    • 1
    AA 1-249

    原理

    Anti-NIT2 Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-NIT2 Antibody Picoband® (ABIN7599155). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human NIT2 recombinant protein (Position: M1-K249).

    亚型

    IgG
  • 应用备注

    Western blot, 0.25-0.5 μg/mL, Human, Rat
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Asakura, M., Nakano, M., Hayashida, K., Fujii, H., Nakajima, M., Atsuda, K., Itoh, T., Fujiwara, R. Human nitrilase-like protein does not catalyze the hydrolysis of vildagliptin. Drug Metab. Pharmacokinet. 29: 463-469, 2014. 2. Chien, C.-H., Gao, Q.-Z., Cooper, A. J. L., Lyu, J.-H., Sheu, S.-Y. Structural insights into the catalytic active site and activity of human Nit2/omega-amidase: kinetic assay and molecular dynamics simulation. J. Biol. Chem. 287: 25715-25726, 2012. 3. Jaisson, S., Veiga-da-Cunha, M., Van Schaftingen, E. Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2. Biochimie 91: 1066-1071, 2009.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    NIT2 (Nitrilase Family, Member 2 (NIT2))

    别名

    NIT2

    背景

    Synonyms: Troponin T, fast skeletal muscle, TnTf, Beta-TnTF, Fast skeletal muscle troponin T, fTnT, TNNT3

    Tissue Specificity: In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.

    Background: NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.

    分子量

    33 kDa

    基因ID

    56954
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