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MED7 抗体 (AA 1-233)

This anti-MED7 antibody is a 兔 多克隆 antibody detecting MED7 in WB, ELISA, ICC 和 IF. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN7599129
发货至: 中国

Quick Overview for MED7 抗体 (AA 1-233) (ABIN7599129)

抗原

See all MED7 抗体
MED7 (Mediator Complex Subunit 7 (MED7))

适用

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人, 小鼠, 大鼠

宿主

  • 41
  • 6

克隆类型

  • 43
  • 4
多克隆

标记

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  • 4
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  • 1
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This MED7 antibody is un-conjugated

应用范围

  • 42
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  • 1
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Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
  • 抗原表位

    • 15
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 1-233

    原理

    Anti-MED7 Antibody Picoband®

    产品特性

    Anti-MED7 Antibody Picoband® (ABIN7599129). Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human MED7 recombinant protein (Position: M1-P233). Human MED7 shares 97% amino acid (aa) sequence identity with mouse MED7.
  • 应用备注

    Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Myers, L. C., Gustafsson, c. M., Bushnell, D. A., Lui, M., Erdjument-Bromage, H., Tempst, P., Kornberg, R. D. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev. 12: 45-54, 1998. 2. Ryu, S., Zhou, S., Ladurner, A. G., Tjian, R. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. Nature 397: 446-450, 1999. 3. Sato, S., Tomomori-Sato, C., Parmely, T. J., Florens, L., Zybailov, B., Swanson, S. K., Banks, C. A. S., Jin, J., Cai, Y., Washburn, M. P., Conaway, J. W., Conaway, R. C. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Molec. Cell 14: 685-691, 2004.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    MED7 (Mediator Complex Subunit 7 (MED7))

    别名

    MED7

    背景

    Mediator of RNA polymerase II transcription subunit 7 is an enzyme that in humans is encoded by the MED7 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.

    基因ID

    9443

    UniProt

    O43513

    途径

    Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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