DDB1 and CUL4-Associated Factor 1 (DCAF1) (AA 1-195) 抗体
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Quick Overview for DDB1 and CUL4-Associated Factor 1 (DCAF1) (AA 1-195) 抗体 (ABIN7599063)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-195
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原理
- Anti-VPRBP/DCAF1 Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-VPRBP/DCAF1 Antibody (ABIN7599063). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human VPRBP/DCAF1 recombinant protein (Position: M1-R195).
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亚型
- IgG
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anti-DDB1 and CUL4-Associated Factor 1 (DCAF1) (AA 647-745) antibody
DCAF1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DDB1 and CUL4-Associated Factor 1 (DCAF1) antibodyDCAF1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB3745 unconjugated Recombinant Antibody
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- DDB1 and CUL4-Associated Factor 1 (DCAF1)
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别名
- DCAF1
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背景
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Synonyms: Protein Bop, BH3-only protein, Retrotransposon Gag-like protein 10, RTL10, BOP, C22orf29
Tissue Specificity: Ubiquitously expressed.
Background: VprBP was first identified as a protein that can interact with HIV-1 viral protein R. It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein. VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells. Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway.
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分子量
- 180 kDa
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基因ID
- 9730
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UniProt
- Q9Y4B6
抗原
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