Anti-MED28 Antibody Picoband® (ABIN7599039). Tested in ELISA, Flow Cytometry, IP, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
纯化方法
Immunogen affinity purified.
免疫原
E.coli-derived human MED28 recombinant protein (Position: M1-T178). Human MED28 shares 94.4% and 94.9% amino acid (aa) sequence identity with mouse and rat MED28, respectively.
MED28
适用: 小鼠
IF (cc), IF (p)
宿主: 兔
Polyclonal
Cy5.5
应用备注
Western blot, 0.25-0.5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL, - 1. Brass, A. L., Dykxhoorn, D. M., Benita, Y., Yan, N., Engelman, A., Xavier, R. J., Lieberman, J., Elledge, S. J. Identification of host proteins required for HIV infection through a functional genomic screen. Science 319: 921-926, 2008. 2. Liu, C., Zhang, L., Shao, Z.-M., Beatty, P., Sartippour, M., Lane, T. F., Barsky, S. H., Livingston, E., Nguyen, M. Identification of a novel endothelial-derived gene EG-1. Biochem. Biophys. Res. Commun. 290: 602-612, 2002. 3. Lu, M., Zhang, L., Maul, R. S., Sartippour, M. R., Norris, A., Whitelegge, J., Rao, J. Y., Brooks, M. N. The novel gene EG-1 stimulates cellular proliferation. Cancer Res. 65: 6159-6166, 2005.
限制
仅限研究用
状态
Lyophilized
溶解方式
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
抗原
MED28
(Mediator Complex Subunit 28 (MED28))
别名
MED28
背景
Mediator of RNA polymerase II transcription subunit 28 is an enzyme that in humans is encoded by the MED28 gene. MED28 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNApolymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatoryproteins to the basal RNA polymerase.