MRPS25 抗体 (AA 1-173)
Quick Overview for MRPS25 抗体 (AA 1-173) (ABIN7599032)
抗原
See all MRPS25 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-173
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原理
- Anti-MRPS25 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MRPS25 Antibody Picoband® (ABIN7599032). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human MRPS25 recombinant protein (Position: M1-D173).
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亚型
- IgG
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anti-Mitochondrial Ribosomal Protein S25 (MRPS25) (AA 145-173), (C-Term) antibody
MRPS25 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB24754 unconjugated
anti-Mitochondrial Ribosomal Protein S25 (MRPS25) (C-Term) antibodyMRPS25 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Ribosomal Protein S25 (MRPS25) (C-Term) antibodyMRPS25 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Ribosomal Protein S25 (MRPS25) (AA 1-173) antibodyMRPS25 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Mitochondrial Ribosomal Protein S25 (MRPS25) (N-Term) antibodyMRPS25 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bugiardini, E., Mitchell, A. L., Dalla Rosa, I., Horning-Do, H.-T., Pitmann, A. M., Poole, O. V., Holton, J. L., Shah, S., Woodward, C., Hargreaves, I., Quinlivan, R., Amunts, A., Wiesner, R. J., Houlden, H., Holt, I. J., Hanna, M. G., Pitceathly, R. D. S., Spinazzola, A. MRPS25 mutations impair mitochondrial translation and cause encephalomyopathy. Hum. Molec. Genet. 28: 2711-2719, 2019. 2. Kenmochi, N., Suzuki, T., Uechi, T., Magoori, M., Kuniba, M., Higa, S., Watanabe, K., Tanaka, T. The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders. Genomics 77: 65-70, 2001. 3. Koc, E. C., Burkhart, W., Blackburn, K., Moseley, A., Spremulli, L. L. The small subunit of the mammalian mitochondrial ribosome: identification of the full complement of ribosomal proteins present. J. Biol. Chem. 276: 19363-19374, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- MRPS25 (Mitochondrial Ribosomal Protein S25 (MRPS25))
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别名
- MRPS25
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背景
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Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,
Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .
Background: 28S ribosomal protein S25, mitochondrial is a protein that in humans is encoded by the MRPS25 gene. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.
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分子量
- 20 kDa
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基因ID
- 64432
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UniProt
- P82663
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途径
- SARS-CoV-2 Protein Interactome
抗原
-
