Vinculin 抗体 (AA 1-149)
Quick Overview for Vinculin 抗体 (AA 1-149) (ABIN7599003)
抗原
See all Vinculin (VCL) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-149
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原理
- Anti-Vinculin/VCL Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Vinculin/VCL Antibody Picoband® (ABIN7599003). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Vinculin/VCL recombinant protein (Position: M1-E149).
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亚型
- IgG
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anti-Vinculin (VCL) (AA 1-1066) antibody
VCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C6-1B5 unconjugated
anti-Vinculin (VCL) (AA 879-1064) antibodyVCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) (AA 903-937) antibodyVCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55983 unconjugated
anti-Vinculin (VCL) (AA 1020-1134) antibodyVCL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) (AA 634-668) antibodyVCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55982 unconjugated
anti-Vinculin (VCL) (AA 1-1066) antibodyVCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F8-1D4 unconjugated
anti-Vinculin (VCL) (AA 174-322) antibodyVCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2575 unconjugated
anti-Vinculin (VCL) (AA 174-322) antibodyVCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2572 unconjugated
anti-Vinculin (VCL) (AA 767-1066) antibodyVCL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) antibodyVCL 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bakolitsa, C., Cohen, D. M., Bankston, L. A., Bobkov, A. A., Cadwell, G. W., Jennings, L., Critchley, D. R., Craig, S. W., Liddington, R. C. Structural basis for vinculin activation at sites of cell adhesion. Nature 430: 583-586, 2004. 2. del Rio, A., Perez-Jimenez, R., Liu, R., Roca-Cusachs, P., Fernandez, J. M., Sheetz, M. P. Stretching single talin rod molecules activates vinculin binding. Science 323: 638-641, 2009. 3. Gimona, M., Small, J. V., Moeremans, M., Van Damme, J., Puype, M., Vandekerckhove, J. Porcine vinculin and metavinculin differ by a 68-residue insert located close to the carboxy-terminal part of the molecule. EMBO J. 7: 2329-2334, 1988. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Vinculin (VCL)
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别名
- VCL
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背景
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Synonyms: Vinculin, Metavinculin, MV, VCL
Tissue Specificity: Metavinculin is muscle-specific.
Background: In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. It is mapped to 10q22.2. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
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分子量
- 124 kDa
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基因ID
- 7414
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UniProt
- P18206
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途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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