TKT 抗体 (AA 1-116)
Quick Overview for TKT 抗体 (AA 1-116) (ABIN7598958)
抗原
See all TKT 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-116
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原理
- Anti-Transketolase/TKT Picoband® Antibody (monoclonal, 3E5)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Transketolase/TKT Picoband® Antibody (monoclonal, 3E5) (ABIN7598958). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Transketolase/TKT recombinant protein (Position: M1-A116).
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亚型
- IgG2a
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anti-Transketolase (TKT) (AA 1-294) antibody
TKT 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transketolase (TKT) (AA 1-286) antibodyTKT 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transketolase (TKT) antibodyTKT 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transketolase (TKT) (AA 1-280) antibodyTKT 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transketolase (TKT) antibodyTKT 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transketolase (TKT) (AA 1-623) antibodyTKT 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Transketolase (TKT) (N-Term) antibodyTKT 适用: 人, 大鼠, 小鼠, Cow, Pig, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transketolase (TKT) antibodyTKT 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transketolase (TKT) (AA 1-623) antibodyTKT 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Transketolase (TKT) antibodyTKT 适用: 人, 大鼠, 小鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Abedinia, M., Layfield, R., Jones, S. M., Nixon, P. F., Mattick, J. S. Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase. Biochem. Biophys. Res. Commun. 183: 1159-1166, 1992. 2. Boyle, L., Wamelink, M. M. C., Salomons, G. S., Roos, B., Pop, A., Dauber, A., Hwa, V., Andrew, M., Douglas, J., Feingold, M., Kramer, N., Saitta, S., Retterer, K., Cho, M. T., Begtrup, A., Monaghan, K. G., Wynn, J., Chung, W. K. Mutations in TKT are the cause of a syndrome, including short stature, developmental delay, and congenital heart defects. Am. J. Hum. Genet. 98: 1235-1242, 2016. 3. Coy, J. F., Dubel, S., Kioschis, P., Thomas, K., Micklem, G., Delius, H., Poustka, A. Molecular cloning of tissue-specific transcripts of a transketolase-related gene: implications for the evolution of new vertebrate genes. Genomics 32: 309-316, 1996. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- TKT (Transketolase (TKT))
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别名
- TKT
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背景
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Synonyms: Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK
Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.
Background: Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is ly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.
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分子量
- 68 kDa
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基因ID
- 7086
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UniProt
- P29401
抗原
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